Oriented Neural Spheroid Formation and Differentiation of Neural Stem Cells Guided by Anisotropic Inverse Opals
Oriented Neural Spheroid Formation and Differentiation of Neural Stem Cells Guided by Anisotropic Inverse Opals
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各向异性反蛋白石引导神经球体定向形成和神经干细胞分化
DOI：
10.3389/fbioe.2020.00848
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发表时间：
2020-07
影响因子：
5.7
通讯作者：
Chai Renjie
中科院分区：
文献类型：
--
作者：
Xia Lin;Shang Yixuan;Chen Xiangbo;Li He;Xu Xiaochen;Liu Wei;Yang Guang;Wang Tian;Gao Xia;Chai Renjie
各向同性反蛋白石结构已被广泛研究的能力，操纵细胞的行为，如附着，迁移，和球体的形成。然而，它们在调节神经干细胞行为方面的应用尚未得到充分的探索，此外，各向同性的反蛋白石结构通常缺乏诱导细胞定向生长的能力，而定向生长是基于神经干细胞治疗的神经再生的基础。通过对聚偏氟乙烯（PVDF）反蛋白石薄膜进行机械拉伸，得到了各向异性反蛋白石衬底。各向异性反蛋白石基质具有良好的生物相容性、光学特性和各向异性，对神经球的形成、神经干细胞的定向排列、神经干细胞的分化、衍生神经元的定向生长和新生神经元树突的复杂性具有良好的指导作用。因此，我们得出结论，各向异性反蛋白石基板具有巨大的潜力，在神经再生的应用。
Isotropic inverse opal structures have been extensively studied for the ability to manipulate cell behaviors such as attachment, migration, and spheroid formation. However, their use in regulate the behaviors of neural stem cells has not been fully explored, besides, the isotropic inverse opal structures usually lack the ability to induce the oriented cell growth which is fundamental in neural regeneration based on neural stem cell therapy. In this paper, the anisotropic inverse opal substrates were obtained by mechanically stretching the poly (vinylidene fluoride) (PVDF) inverse opal films. The anisotropic inverse opal substrates possessed good biocompatibility, optical properties and anisotropy, provided well guidance for the formation of neural spheroids, the alignment of neural stem cells, the differentiation of neural stem cells, the oriented growth of derived neurons and the dendritic complexity of the newborn neurons. Thus, we conclude that the anisotropic inverse opal substrates possess great potential in neural regeneration applications.
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影响因子：
4.6
作者：
McGinley LM;Kashlan ON;Bruno ES;Chen KS;Hayes JM;Kashlan SR;Raykin J;Johe K;Murphy GG;Feldman EL
通讯作者：
Feldman EL
DOI：
10.1007/978-3-319-56637-5_72-1
发表时间：
2019-12
期刊：
Drug Discovery and Evaluation: Methods in Clinical Pharmacology
影响因子：
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作者：
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通讯作者：
N. Graffmann;Lucas-Sebastian Spitzhorn;Soraia Martins;Shaifur Rahman;Lisa Nguyen;James Adjaye
DOI：
10.1007/springerreference_36865
发表时间：
2019
期刊：
Introduction to Basics of Pharmacology and Toxicology
影响因子：
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作者：
P. Devaraju;D. Kaliyaperumal;T. Madhavan
通讯作者：
P. Devaraju;D. Kaliyaperumal;T. Madhavan
影响因子：
9.5
作者：
Jiang, Ziyun;Song, Qin;Cheng, Guosheng
通讯作者：
Cheng, Guosheng
层粘连蛋白涂层电纺再生丝素蛋白垫促进神经祖细胞体外增殖、分化和存活
影响因子：
5.7
作者：
Li, Guangfei;Chen, Kai;Sun, Shan
通讯作者：
Sun, Shan