Recovery of lung pyridine nucleotides following acute exposure of adult and aged rats to ozone.
Recovery of lung pyridine nucleotides following acute exposure of adult and aged rats to ozone.
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成年和老年大鼠急性暴露于臭氧后肺吡啶核苷酸的恢复。
DOI：
10.1080/15287399109531552
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Balis,JU
中科院分区：
文献类型：
--
作者：
Montgomery,MR;Raska-Emery,P;Balis,JU
将6月龄和24月龄的雄性无病原体Fischer 344大鼠暴露于1.5或3.0 ppm，持续8 h，测量二磷酸核苷酸（NAD+和NADH）和三磷酸核苷酸（NADP+和NADPH）的回收率，并与对照组进行比较。未检查0.5 ppm后的恢复情况，因为在此较低暴露量后，两个年龄组均未发生显著变化。在零时（暴露后立即），成年和老年动物中的两种浓度均降低，但老年动物中3.0 ppm的NADH除外;成年动物中1.5和3.0 ppm的NADP+降低，但不显著。对于NAD+和NADH，在暴露于1.5或3.0 ppm臭氧8 h后，全肺浓度在24 h内完全恢复。仅在3.0 ppm臭氧后，还原型与氧化型的比率（NADH/NAD+）在24 h后仍升高;然而，到96 h时也恢复到对照水平。对于三磷酸核苷酸，暴露于1.5 ppm臭氧8 h导致整个96 h恢复期内NADPH的全肺浓度持续下降。此外，仅在1.5 ppm暴露后，还原氧化比（NADPH/NADP+）在整个96 h恢复期内显著降低。出乎意料的是，在暴露于1.5和3.0 ppm浓度8 h后，全肺水平在24 h内恢复正常。除了对NADPH水平的持续影响外，这些数据表明，在重度急性氧化损伤后，肺中的二磷酸核苷酸和三磷酸核苷酸浓度迅速恢复正常。成人组和老年组的恢复率无差异。
Male, pathogen‐free Fischer 344 rats aged 6 and 24 mo were exposed to 1.5 or 3.0 ppm for 8 h and recovery rates of diphosphonucleotides (NAD+and NADH) and triphosphonucleotides (NADP+and NADPH) were measured and compared to controls. Recovery after 0.5 ppm was not examined because no significant changes occurred in either age group after this lower exposure. At zero time (immediately after exposures) both concentrations are depressed in adults and aged animals except for NADH in aged animals at 3.0 ppm; NADP+in adults at 1.5 and 3.0 ppm was decreased, but not significantly. For NAD+and NADH, recovery of whole lung concentrations is complete by 24 h following an 8‐h exposure to 1.5 or 3.0 ppm of ozone. Only after 3.0 ppm of ozone was the ratio of the reduced to oxidized form (NADH/NAD+) still elevated after 24 h; however, it also returned to control levels by 96 h. For the triphosphonucleotides, an 8‐h exposure to 1.5 ppm of ozone resulted in a sustained depression of whole lung concentrations of NADPH throughout the 96‐h recovery period. Also, only after the 1.5 ppm exposure was the reduced to oxidized ratio (NADPH/NADP+) significantly depressed throughout the 96‐h recovery period. Unexpectedly, recovery of whole lung levels returned to normal within 24 h after the 8‐h exposure to both the 1.5 and the 3.0 ppm concentrations. With the exception of the sustained effect on NADPH levels, these data indicate that di‐ and triphosphonucleotide concentrations rapidly return to normal in the lung after severe, acute oxidant injury. There were no differences in recovery rates between the adult and the aged groups.
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影响因子：
10.4
作者：
通讯作者：
--
暴露于臭氧的大鼠肺细胞群的谷胱甘肽途径酶活性和臭氧敏感性。
影响因子：
4.5
作者：
I. Rietjens;L. van Bree;M. Marra;M. Poelen;P. Rombout;G. Alink
通讯作者：
G. Alink
臭氧的细胞、生化和功能影响：臭氧健康影响的新研究和观点。
影响因子：
3.5
作者：
E. S. Wright;D. Dziedzic;C. Wheeler
通讯作者：
C. Wheeler
臭氧引起的损伤的病理学。
DOI：
--
发表时间：
1990
期刊：
Archives of Environmental Health An International Journal
影响因子：
--
作者：
J. Steinberg;J. Gleeson;D. Gil
通讯作者：
D. Gil
大鼠肝脏和其他组织中烟酰胺腺嘌呤二核苷酸的测定程序。
DOI：
10.3109/13813456409065351
发表时间：
1964
期刊：
Archives internationales de physiologie et de biochimie
影响因子：
--
作者：
T. Slater;B. Sawyer;U. Sträuli
通讯作者：
U. Sträuli