Epitope mapping and identification of amino acids critical for mouse IgG-binding to linear epitopes on Gly m Bd 28K
Epitope mapping and identification of amino acids critical for mouse IgG-binding to linear epitopes on Gly m Bd 28K
复制标题
表位作图和鉴定对于小鼠 IgG 与 Gly m Bd 28K 上的线性表位结合至关重要的氨基酸
DOI：
10.1080/09168451.2016.1165604
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发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Huili Yan
中科院分区：
文献类型：
--
作者：
Jun Xi;Huili Yan
Gly m Bd 28 K是大豆中的主要过敏原之一，但关于其IgG结合表位的信息有限。合成了34个覆盖Gly m Bd 28 K全序列的重叠肽，并利用3个抗Gly m Bd 28 K的单克隆抗体鉴定了Gly m Bd 28 K的IgG结合区。识别了对应于28 GDKKSPKSLFLMSNS 42（G28-S42）、56 LKSHGGRIFYRHMHI 70（L56-I70）和154 ETKSFYIGGGANSH 168（E154-H168）的三个优势肽。L56-I70是Gly m Bd 28 K蛋白的最重要表位，竞争性ELISA结果表明，L56-I70能抑制单克隆抗体与Gly m Bd 28 K蛋白的结合。L56-I70的丙氨酸扫描证明F64、Y 65和R66是该表位的关键氨基酸。用ABCpred和BepiPred两种生物信息学工具对Gly m Bd 28 K的表位进行预测，并与单克隆抗体定位的表位进行比较。表位作图和识别小鼠IgG与Gly m Bd 28 K线性表位结合的关键氨基酸。
Gly m Bd 28K is one of the major allergens in soybeans, but there is limited information on its IgG-binding epitopes. Thirty-four overlapping peptides that covered the entire sequence of Gly m Bd 28K were synthesized, and 3 monoclonal antibodies against Gly m Bd 28K were utilized to identify the IgG-binding regions of Gly m Bd 28K. Three dominant peptides corresponding to 28GDKKSPKSLFLMSNS42(G28-S42), 56LKSHGGRIFYRHMHI70(L56-I70), and 154ETFQSFYIGGGANSH168(E154-H168) were recognized. L56-I70 is the most important epitope, and a competitive ELISA indicated that it could inhibit the binding of monoclonal antibody to Gly m Bd 28K protein. Alanine scanning of L56-I70 documented that F64, Y65, and R66 were the critical amino acids of this epitope. Two bioinformatics tools, ABCpred and BepiPred, were used to predict the epitopes of Gly m Bd 28K, and the predictions were compared with the epitopes that we had located by monoclonal antibodies. Graphical abstract Epitope mapping and identification of amino acids critical for mouse IgG-binding to linear epitopes on Gly m Bd 28K.
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影响因子：
3
作者：
Resende DM;Rezende AM;Oliveira NJ;Batista IC;Corrêa-Oliveira R;Reis AB;Ruiz JC
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2000
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DOI：
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