Ovary ecdysteroidogenic hormone functions independently of the insulin receptor in the yellow fever mosquito, Aedes aegypti.
Ovary ecdysteroidogenic hormone functions independently of the insulin receptor in the yellow fever mosquito, Aedes aegypti.
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卵巢的雌激素激素独立于黄热病蚊子中的胰岛素受体，埃及埃及。
DOI：
10.1016/j.ibmb.2013.09.004
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发表时间：
2013-12
影响因子：
3.8
通讯作者：
Strand MR
中科院分区：
文献类型：
--
作者：
Dhara A;Eum JH;Robertson A;Gulia-Nuss M;Vogel KJ;Clark KD;Graf R;Brown MR;Strand MR
大多数蚊子必须以脊椎动物宿主的血液为食才能产卵。在黄热病蚊子，埃及伊蚊，吸血触发大脑中的内侧神经分泌细胞释放胰岛素样肽（ILP）和卵巢蜕皮类固醇激素（OEH）。这些激素随后直接诱导卵巢产生蜕皮类固醇激素（ECD），其激活脂肪体中卵黄蛋白的合成以供卵母细胞摄取。ILP3通过与蚊子胰岛素受体（MIR）结合来刺激ECD产生。相反，关于OEH的作用模式知之甚少，OEH是称为neuroparsin的神经肽家族的成员。在这里，我们报告说，OEH是唯一的neuroparsin家族成员存在于Ae。埃及蚊子基因组，其它蚊子也只编码一种neuroparsin基因。免疫印迹实验表明，全长形式的肽，我们称之为长OEH（lOEH），被加工成短OEH（sOEH）。然而，加工的重要性仍然不清楚，因为重组形式的lOEH（rlOEH）和合成的sOEH表现出非常相似的生物活性。一系列实验表明，rlOEH和sOEH都不与ILP3或MIR结合。信号传导研究进一步显示ILP3激活MIR，但rlOEH不激活，然而两种神经肽都激活Akt，Akt是胰岛素途径信号传导的标志物。我们的结果还表明，卵巢中TOR信号传导的激活需要氨基酸和ILP3或rlOEH的共刺激。总之，我们得出结论，OEH激活胰岛素信号通路独立于MIR，胰岛素和TOR信号在卵巢耦合。
Most mosquito species must feed on the blood of a vertebrate host to produce eggs. In the yellow fever mosquito, Aedes aegypti, blood feeding triggers medial neurosecretory cells in the brain to release insulin-like peptides (ILPs) and ovary ecdysteroidogenic hormone (OEH). Theses hormones thereafter directly induce the ovaries to produce ecdysteroid hormone (ECD), which activates the synthesis of yolk proteins in the fat body for uptake by oocytes. ILP3 stimulates ECD production by binding to the mosquito insulin receptor (MIR). In contrast, little is known about the mode of action of OEH, which is a member of a neuropeptide family called neuroparsin. Here we report that OEH is the only neuroparsin family member present in the Ae. aegypti genome and that other mosquitoes also encode only one neuroparsin gene. Immunoblotting experiments suggested that the full-length form of the peptide, which we call long OEH (lOEH), is processed into short OEH (sOEH). The importance of processing, however, remained unclear because a recombinant form of lOEH (rlOEH) and synthetic sOEH exhibited very similar biological activity. A series of experiments indicated that neither rlOEH nor sOEH bound to ILP3 or the MIR. Signaling studies further showed that ILP3 activated the MIR but rlOEH did not, yet both neuropeptides activated Akt, which is a marker for insulin pathway signaling. Our results also indicated that activation of TOR signaling in the ovaries required co-stimulation by amino acids and either ILP3 or rlOEH. Overall, we conclude that OEH activates the insulin signaling pathway independently of the MIR, and that insulin and TOR signaling in the ovaries is coupled.
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影响因子：
2.8
作者：
Gulia-Nuss, Monika;Eum, Jai-Hoon;Brown, Mark R.
通讯作者：
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Brown, MR
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Badisco, Liesbeth;Claeys, Ilse;Broeck, Jozef Vanden
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Broeck, Jozef Vanden