Population structure of manganese-oxidizing bacteria in stratified soils and properties of manganese oxide aggregates under manganese-complex medium enrichment.
Population structure of manganese-oxidizing bacteria in stratified soils and properties of manganese oxide aggregates under manganese-complex medium enrichment.
复制标题
DOI：
10.1371/journal.pone.0073778
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Yang W;Zhang Z;Zhang Z;Chen H;Liu J;Ali M;Liu F;Li L
对水环境中的锰氧化细菌进行了较全面的研究。然而，这些细菌在陆地土壤环境中的分布和地球化学意义的信息很少。在这项研究中，初步研究了分层土壤锰氧化细菌的群落结构和多样性。来自所有基质的总共344个可培养细菌分离株在1 µM至240 µM等效MnO 2范围内表现出Mn（II）氧化活性。高锰（II）氧化分离株（>50 mM MnO 2）被鉴定为放线菌门、厚壁菌门和变形菌门的物种。通过比较系统发育分析，发现了7个新的Mn（II）氧化细菌属（种），即埃希氏菌属，土壤杆菌属，纤维单胞菌属，贪铜菌属，微杆菌属，罗尔斯通氏菌属和Variovorax。此外，在土壤细菌群落的多样性增加后，观察锰（II）和富碳复合体的联合富集。富集细菌的系统发育分类的优势变性梯度凝胶电泳带，显然是类似的可培养的锰（II）氧化细菌。进一步进行实验，以调查由具有高Mn（II）氧化活性的细菌分离物形成的氧化锰聚集体的性质。结果表明，这些细菌与他们的锰氧化物紧密结壳，并形成规则的微球聚集体下延长Mn（II）和富碳培养基富集三周。通过动力学检查证实了这些菌株对Mn（II）至Mn（III/IV）的生物氧化。X-射线衍射分析表明，从这些聚集体的几个锰氧化物和菱锰矿的特征峰。亮贝林蓝测试也验证了这些聚集体的Mn（II）氧化活性。这些结果表明，在富集条件下形成一定量的锰氧化物，沿着在这种聚集体中形成菱锰矿。因此，本研究提供了深入了解土壤中的细菌群落的结构和多样性的Mn（II）氧化的栖息地，并支持土壤中的锰氧化物矿化的贡献土壤中的Mn（II）氧化细菌。
Manganese-oxidizing bacteria in the aquatic environment have been comprehensively investigated. However, little information is available about the distribution and biogeochemical significance of these bacteria in terrestrial soil environments. In this study, stratified soils were initially examined to investigate the community structure and diversity of manganese-oxidizing bacteria. Total 344 culturable bacterial isolates from all substrata exhibited Mn(II)-oxidizing activities at the range of 1 µM to 240 µM of the equivalent MnO2. The high Mn(II)-oxidizing isolates (>50 mM MnO2) were identified as the species of phyla Actinobacteria, Firmicutes and Proteobacteria. Seven novel Mn(II)-oxidizing bacterial genera (species), namely, Escherichia, Agromyces, Cellulomonas, Cupriavidus, Microbacterium, Ralstonia, and Variovorax, were revealed via comparative phylogenetic analysis. Moreover, an increase in the diversity of soil bacterial community was observed after the combined enrichment of Mn(II) and carbon-rich complex. The phylogenetic classification of the enriched bacteria represented by predominant denaturing gradient gel electrophoresis bands, was apparently similar to culturable Mn(II)-oxidizing bacteria. The experiments were further undertaken to investigate the properties of the Mn oxide aggregates formed by the bacterial isolates with high Mn(II)-oxidizing activity. Results showed that these bacteria were closely encrusted with their Mn oxides and formed regular microspherical aggregates under prolonged Mn(II) and carbon-rich medium enrichment for three weeks. The biotic oxidation of Mn(II) to Mn(III/IV) by these isolates was confirmed by kinetic examinations. X-ray diffraction assays showed the characteristic peaks of several Mn oxides and rhodochrosite from these aggregates. Leucoberbelin blue tests also verified the Mn(II)-oxidizing activity of these aggregates. These results demonstrated that Mn oxides were formed at certain amounts under the enrichment conditions, along with the formation of rhodochrosite in such aggregates. Therefore, this study provides insights into the structure and diversity of soil-borne bacterial communities in Mn(II)-oxidizing habitats and supports the contribution of soil-borne Mn(II)-oxidizing bacteria to Mn oxide mineralization in soils.
登录
查看更多内容
影响因子：
4.4
作者：
Francis, CA;Co, EM;Tebo, BM
通讯作者：
Tebo, BM
DOI：
10.1007/bf01611203
发表时间：
1973-01-01
期刊：
HELGOLANDER WISSENSCHAFTLICHE MEERESUNTERSUCHUNGEN
影响因子：
--
作者：
KRUMBEIN, WE;ALTMANN, HJ
通讯作者：
ALTMANN, HJ
磁铁矿和赤铁矿存在下 Mn(II) 的非生物氧化诱导 As(III) 的氧化和移动
影响因子：
13.6
作者：
Ren, Hai-Tao;Jia, Shao-Yi;Han, Xu
通讯作者：
Han, Xu
影响因子：
15
作者：
Kim, HS;Pastén, PA;Stair, PC
通讯作者：
Stair, PC
影响因子：
5.1
作者：
Lee, Ji-Hoon;Kennedy, David W.;Fredrickson, James K.
通讯作者：
Fredrickson, James K.