Bub1 is not required for the checkpoint response to unattached kinetochores in diploid human cells
Bub1 is not required for the checkpoint response to unattached kinetochores in diploid human cells
复制标题
Bub1 不是对二倍体人类细胞中未附着动粒的检查点反应所必需的
DOI：
10.1101/278820
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Currie C
中科院分区：
文献类型：
--
作者：
Currie C
在有丝分裂过程中，无错误的染色体分离依赖于功能性纺锤体组装检查点（SAC）。SAC是一种多组分信号传导系统，其被募集到不正确连接的着丝粒以催化形成可溶性抑制剂，称为有丝分裂检查点复合物（MCC），其结合并抑制后期促进复合物。我们以前提出，两个可分离的途径，由KNL 1-Bub 3-Bub 1（KBB）和Rod-Zwilch-Zw 10（RZZ）组成，招募Mad 1-Mad 2复合物到人类动粒激活SAC。我们称之为双通道模型。尽管Bub 1是酵母（缺乏RZZ）中MCC形成所必需的，但基于siRNA研究，关于人类细胞中是否也是这种情况，存在相互矛盾的证据[-]。在这里，我们报告，使用基因组编辑，Bub 1不是严格要求SAC响应于人类二倍体hTERT-RPE 1细胞中未连接的动粒，与双途径模型一致。
Error-free chromosome segregation during mitosis depends on a functional spindle assembly checkpoint (SAC). The SAC is a multi-component signaling system that is recruited to incorrectly attached kinetochores to catalyze the formation of a soluble inhibitor, known as the mitotic checkpoint complex (MCC), which binds and inhibits the anaphase promoting complex . We have previously proposed that two separable pathways, composed of KNL1-Bub3-Bub1 (KBB) and Rod-Zwilch-Zw10 (RZZ), recruit Mad1-Mad2 complexes to human kinetochores to activate the SAC . We refer to this as the dual pathway model. Although Bub1 is absolutely required for MCC formation in yeast (which lack RZZ), there is conflicting evidence as to whether this is also the case in human cells based on siRNA studies [–]. Here we report, using genome editing, that Bub1 is not strictly required for the SAC response to unattached kinetochores in human diploid hTERT-RPE1 cells, consistent with the dual pathway model.
ABBA基序结合APC/C激活剂，并由APC/C底物和调节器共享。
影响因子：
11.8
作者：
Di Fiore B;Davey NE;Hagting A;Izawa D;Mansfeld J;Gibson TJ;Pines J
通讯作者：
Pines J
影响因子：
11.8
作者：
Meraldi, P;Draviam, VM;Sorger, PK
通讯作者：
Sorger, PK