Non-Invasive Multiphoton Imaging of Islets Transplanted Into the Pinna of the NOD Mouse Ear Reveals the Immediate Effect of Anti-CD3 Treatment in Autoimmune Diabetes.
Non-Invasive Multiphoton Imaging of Islets Transplanted Into the Pinna of the NOD Mouse Ear Reveals the Immediate Effect of Anti-CD3 Treatment in Autoimmune Diabetes.
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DOI：
10.3389/fimmu.2018.01006
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发表时间：
2018
影响因子：
7.3
通讯作者：
Wållberg M
中科院分区：
文献类型：
--
作者：
Benson RA;Garcon F;Recino A;Ferdinand JR;Clatworthy MR;Waldmann H;Brewer JM;Okkenhaug K;Cooke A;Garside P;Wållberg M
我们提出了一种新的和容易获得的方法，促进细胞的时间分辨成像的移植胰岛。将胰岛移植到小鼠耳廓允许对胰岛中的事件进行非侵入性的体内纵向成像，并且能够改善实验数据的采集，并且使用比使用侵入性技术可能使用的实验动物更少的实验动物，因为可以在免疫干预之前和之后评估相同小鼠的胰岛浸润细胞的存在。我们已经应用这种方法通过抗CD3注射的良好使用来研究β细胞的治疗保护，并且已经获得了关于对胰岛浸润T细胞的作用的性质和速度的前所未有的数据。我们证明了抗CD3抗体的输注导致对耳耳廓中胰岛移植物中的胰岛浸润T细胞的直接影响，并导致它们在输注20分钟内增加其速度和位移。该技术克服了与胰腺免疫应答的活体成像相关的若干技术挑战，并促进了健康和疾病中β胰岛细胞发育、分化和功能的常规研究。
We present a novel and readily accessible method facilitating cellular time-resolved imaging of transplanted pancreatic islets. Grafting of islets to the mouse ear pinna allows non-invasive, in vivo longitudinal imaging of events in the islets and enables improved acquisition of experimental data and use of fewer experimental animals than is possible using invasive techniques, as the same mouse can be assessed for the presence of islet infiltrating cells before and after immune intervention. We have applied this method to investigating therapeutic protection of beta cells through the well-established use of anti-CD3 injection, and have acquired unprecedented data on the nature and rapidity of the effect on the islet infiltrating T cells. We demonstrate that infusion of anti-CD3 antibody leads to immediate effects on islet infiltrating T cells in islet grafts in the pinna of the ear, and causes them to increase their speed and displacement within 20 min of infusion. This technique overcomes several technical challenges associated with intravital imaging of pancreatic immune responses and facilitates routine study of beta islet cell development, differentiation, and function in health and disease.
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影响因子：
3.7
作者：
Chen BJ;Jiao Y;Zhang P;Sun AY;Pitt GS;Deoliveira D;Drago N;Ye T;Liu C;Chao NJ
通讯作者：
Chao NJ
DOI：
10.1084/jem.20061890
发表时间：
2007-02-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Boissonnas A;Fetler L;Zeelenberg IS;Hugues S;Amigorena S
通讯作者：
Amigorena S
影响因子：
64.8
作者：
Bettelli, E;Carrier, YJ;Kuchroo, VK
通讯作者：
Kuchroo, VK
影响因子：
3.5
作者：
Ambery, P.;Donner, T. W.;Dayan, C. M.
通讯作者：
Dayan, C. M.
影响因子：
7.7
作者：
Chen, Chunguang;Chmelova, Helena;Speier, Stephan
通讯作者：
Speier, Stephan