Long-term in vivo imaging of multiple organs at the single cell level.
Long-term in vivo imaging of multiple organs at the single cell level.
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DOI：
10.1371/journal.pone.0052087
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Chao NJ
中科院分区：
文献类型：
--
作者：
Chen BJ;Jiao Y;Zhang P;Sun AY;Pitt GS;Deoliveira D;Drago N;Ye T;Liu C;Chao NJ
双光子显微镜使研究活体动物的个体细胞行为成为可能。许多器官和组织不能被研究，特别是纵向上，因为它们位于太深的地方，在骨骼结构后面，或者太靠近肺和心脏。在这里，我们报告了一种新的小鼠模型，它允许对许多器官进行长期的单细胞成像。各种各样的活组织成功地植入了小鼠耳朵的耳廓。其中许多植入的组织保持了正常的组织学。以心脏和胸腺为模型，我们进一步证明了移植组织的功能与预期的一样。结合双光子显微镜和荧光示踪剂，我们在几个月的时间里成功地在活体小鼠身上显示了单细胞水平的移植组织。通过该成像获得单个细胞的四维(三维(3D)加时间)信息。该模型使得对多个器官的长期高分辨率4D成像成为可能。
Two-photon microscopy has enabled the study of individual cell behavior in live animals. Many organs and tissues cannot be studied, especially longitudinally, because they are located too deep, behind bony structures or too close to the lung and heart. Here we report a novel mouse model that allows long-term single cell imaging of many organs. A wide variety of live tissues were successfully engrafted in the pinna of the mouse ear. Many of these engrafted tissues maintained the normal tissue histology. Using the heart and thymus as models, we further demonstrated that the engrafted tissues functioned as would be expected. Combining two-photon microscopy with fluorescent tracers, we successfully visualized the engrafted tissues at the single cell level in live mice over several months. Four dimensional (three-dimensional (3D) plus time) information of individual cells was obtained from this imaging. This model makes long-term high resolution 4D imaging of multiple organs possible.
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