Detection of bone marrow-derived lung epithelial cells.
Detection of bone marrow-derived lung epithelial cells.
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DOI：
10.1016/j.exphem.2010.04.011
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发表时间：
2010-07
影响因子：
2.6
通讯作者：
Krause, Diane S.
中科院分区：
文献类型：
--
作者：
Kassmer, Susannah H.;Krause, Diane S.
近十年来，关于骨髓来源细胞在体内适应上皮细胞形态和蛋白表达能力的研究迅速发展，已有数百篇文献报道，骨髓来源细胞可以成为肺、肝、胃肠道、皮肤、胰腺等多个器官的上皮细胞。在这篇综述中，我们对骨髓来源的细胞作为上皮细胞在肺内植入的相关文献进行了严格的评价。目前已发表了40多篇关于骨髓细胞能否分化为肺上皮细胞的手稿，几乎所有的手稿都声称鉴定了骨髓来源的上皮细胞。一些研究已经得出结论，这种细胞并不存在，而且骨髓来源的上皮细胞的现象是基于检测到的人工制品。在这里，我们讨论了已发表的鉴定骨髓来源的上皮细胞的论文中存在的问题，并提出了提供最明确数据的检测方法的标准。骨髓来源上皮细胞的鉴定需要可靠和灵敏的检测技术，这必须包括基于是否存在上皮标记物和缺乏血细胞标记物的细胞鉴定以及供体骨髓来源的标记物。为了使这些研究严谨，他们还必须使用显微镜或单细胞分离排除细胞重叠的方法。一旦这些鉴定骨髓来源的上皮细胞的严格标准被普遍使用，那么该领域就可以向前发展，以解决以下关键问题：哪些骨髓来源的细胞负责作为上皮细胞植入，发生这种情况的机制，这些细胞是否在正常组织修复中发挥作用，以及特定的细胞亚群是否可以用于治疗。
Studies on the ability of bone marrow derived cells to adopt the morphology and protein expression of epithelial cells in vivo have expanded rapidly over the last decade, and hundreds of publications report that bone marrow derived cells can become epithelial cells of multiple organs including lung, liver, GI tract, skin, pancreas and others. In this review, we critically evaluate the literature related to engraftment of bone marrow derived cells as epithelial cells in the lung. Over 40 manuscripts focused on whether bone marrow cells can differentiate into lung epithelial cells have been published, nearly all of which claim to identify marrow derived epithelial cells. A few investigations have concluded that no such cells are present and that the phenomenon of marrow derived epithelial cells is based on detection artifacts. Here we discuss the problems that exist in published papers identifying marrow derived epithelial cells, and propose standards for detection methods that provide the most definitive data. Identification of BM derived epithelial cells requires reliable and sensitive techniques for their detection, which must include cell identification based on the presence of an epithelial marker and the absence of blood cell markers as well as a marker for donor BM origin. In order for these studies to be rigorous, they must also use approaches to rule out cell overlap by microscopy or single cell isolation. Once these stringent criteria for identification of marrow derived epithelial cells are used universally, then the field can move forward to address the critical questions regarding which bone marrow derived cells are responsible for engraftment as epithelial cells, the mechanisms by which this occurs, whether these cells play a role in normal tissue repair, and whether specific cell subsets can be used for therapeutic benefit.
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影响因子：
5.8
作者：
Lee SH;Jang AS;Kim YE;Cha JY;Kim TH;Jung S;Park SK;Lee YK;Won JH;Kim YH;Park CS
通讯作者：
Park CS
DOI：
10.1164/rccm.200902-0242oc
发表时间：
2009-12-01
影响因子：
24.7
作者：
Aslam, Muhammad;Baveja, Rajiv;Kourembanas, Stella
通讯作者：
Kourembanas, Stella
DOI：
10.1165/rcmb.2002-0056rc
发表时间：
2002-12-01
影响因子：
6.4
作者：
Grove, JE;Lutzko, C;Krause, DS
通讯作者：
Krause, DS
DOI：
10.1073/pnas.95.4.1579
发表时间：
1998-02-17
影响因子：
11.1
作者：
Haller, T;Ortmayr, J;Dietl, P
通讯作者：
Dietl, P
影响因子：
4
作者：
MacPherson, H;Keir, P;Dorin, J
通讯作者：
Dorin, J