Splitting culture medium by air-jet and rewetting for the assessment of the wettability of cultured epithelial cell surfaces.
Splitting culture medium by air-jet and rewetting for the assessment of the wettability of cultured epithelial cell surfaces.
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通过空气喷射和再润湿来分裂培养基，以评估培养的上皮细胞表面的润湿性。
DOI：
10.1016/j.biomaterials.2013.08.029
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发表时间：
2013
期刊：
影响因子：
14
通讯作者：
T. Okano
中科院分区：
文献类型：
--
作者：
N. Tanaka;M. Kondo;R. Uchida;M. Kaneko;H. Sugiyama;M. Yamato;T. Okano
本研究发现，大鼠口腔黏膜上皮细胞在不同的培养条件下，挤压培养液后的再润湿现象有所不同。当通过空气喷射应用挤压覆盖在培养细胞上的培养基时，能够通过使用可商购的电影摄像机观察挤压的培养基的运动。在含胎牛血清的角质形成细胞培养液中培养的细胞的挤压宽度是不含胎牛血清的角质形成细胞培养液的1/6。这一结果与两种情况下培养细胞的粘膜层染色状态相对应;在KCM中为阳性，在无FBS培养基中为阴性。此外，在KCM中培养的细胞的粘液糖蛋白MUC 4的基因表达是在无FBS培养基中的100倍，MUC 4蛋白的表达仅显示在细胞的顶端表面。MUC 1、13、15和16在正常和无FBS培养基中的相对基因表达水平被发现不超过KCM中MUC 4的三十分之一。KCM和无FBS培养基之间润湿性差异的主要因素被推测为两种培养基之间MUC 4表达的差异。该方法不仅可以用于测试培养细胞的表面润湿性，而且可以用于测试培养细胞的粘液形成，是一种简单的技术。
This study found that the phenomenon of rewetting after squeezing culture medium varied in different culture conditions for rat oral mucosal epithelial cells. When culture medium covering over cultured cells was squeezed by an air-jet application, the motion of squeezed culture medium was able to be observed by using a commercially available movie camera. Squeezed width on cells cultured in keratinocyte culture medium (KCM), which contained with fetal bovine serum, was one-sixth of that in FBS-free KCM. This result corresponded to the mucous layer staining statuses of cultured cells in both cases; positive in KCM and negative in FBS-free medium. Furthermore, the gene expression of mucous glycoprotein MUC4 in KCM was 100 times higher than that in FBS-free medium, and the expression of MUC4 protein only showed on the apical surface of cells cultured in KCM. The relative gene expression levels of MUC1, 13, 15, and 16 in both the normal and FBS-free medium were found to be no more than one-thirtieth of that of MUC4 in KCM. The main factor of the wettability difference between KCM and FBS-free medium was speculated to be the difference of MUC4 expression between both media. This method can be a simple technique for testing not only the surface wettability but also the mucous formation of cultured cells.
影响因子：
4.4
作者：
Nichols,BA;Chiappino,ML;Dawson,CR
通讯作者：
Dawson,CR
影响因子：
4.4
作者：
Gumus, Koray;Crockett, Charlene Hong;Pflugfelder, Stephen C.
通讯作者：
Pflugfelder, Stephen C.
体外聚合物基质上兔角膜上皮细胞生长的定量。
影响因子：
4.4
作者：
Pettit,DK;Horbett,TA;Hoffman,AS;Chan,KY
通讯作者：
Chan,KY