A Heteromeric RNA-Binding Protein Is Involved in Maintaining Acrophase and Period of the Circadian Clock1[W]
A Heteromeric RNA-Binding Protein Is Involved in Maintaining Acrophase and Period of the Circadian Clock1[W]
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一种异聚 RNA 结合蛋白参与维持昼夜节律钟的末期和周期1[W]
作者：
Dobromir Iliev;Olga Voytsekh;E. Schmidt;Monika Fiedler;A. Nykytenko;M. Mittag
来自莱茵衣藻的rna结合蛋白CHLAMY1由两个亚基组成。一个（命名为C1）含有三个赖氨酸同源基序，另一个（命名为C3）有三个RNA识别基序。CHLAMY1特异性结合尿嘌呤-鸟嘌呤重复序列，其昼夜节律结合活性在翻译后水平受到控制，可能是由C1和C3或单独C1组成的蛋白质复合物的时间依赖性形成。在这里，我们通过测量C1或C3被上调或下调的菌株的趋光性昼夜节律，表征了这两个亚基在昼夜节律系统中的作用。此外，我们还测量了C1或C3水平降低的菌株中亚硝酸盐还原酶活性的节律。当C3水平发生变化（增加或减少）时，趋光性节律和亚硝酸盐还原酶节律（C3减少）的顶相从主观的白天（野生型细胞最大）向夜间移动了几个小时。相比之下，C1的沉默和过表达都会导致昼夜节律紊乱和心律失常。有趣的是，C1的表达式和C3的表达式是相互联系的。我们的数据表明，CHLAMY1参与了莱茵草生物钟的相位角和周期的控制。
The RNA-binding protein CHLAMY1 from the green alga Chlamydomonas reinhardtii consists of two subunits. One (named C1) contains three lysine homology motifs and the other (named C3) has three RNA recognition motifs. CHLAMY1 binds specifically to uridine-guanine-repeat sequences and its circadian-binding activity is controlled at the posttranslational level, presumably by time-dependent formation of protein complexes consisting of C1 and C3 or C1 alone. Here we have characterized the role of the two subunits within the circadian system by measurements of a circadian rhythm of phototaxis in strains where C1 or C3 are either up- or down-regulated. Further, we have measured the rhythm of nitrite reductase activity in strains with reduced levels of C1 or C3. In case of changes in the C3 level (both increases and decreases), the acrophase of the phototaxis rhythm and of the nitrite reductase rhythm (C3 decrease) was shifted by several hours from subjective day (maximum in wild-type cells) back towards the night. In contrast, both silencing and overexpression of C1 resulted in disturbed circadian rhythms and arrhythmicity. Interestingly, the expression of C1 is interconnected with that of C3. Our data suggest that CHLAMY1 is involved in the control of the phase angle and period of the circadian clock in C. reinhardtii.
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DOI：
10.1073/pnas.86.1.172
发表时间：
1989-01-01
影响因子：
11.1
作者：
MORSE, D;MILOS, PM;HASTINGS, JW
通讯作者：
HASTINGS, JW
影响因子：
56.9
作者：
Ishiura, M;Kutsuna, S;Kondo, T
通讯作者：
Kondo, T
影响因子：
10.5
作者：
Elvin, M;Loros, JJ;Heintzen, C
通讯作者：
Heintzen, C
影响因子：
56.9
作者：
Lee, K;Loros, JJ;Dunlap, JC
通讯作者：
Dunlap, JC
DOI：
10.1073/pnas.90.12.5672
发表时间：
1993-06-15
影响因子：
11.1
作者：
KONDO, T;STRAYER, CA;JOHNSON, CH
通讯作者：
JOHNSON, CH