Macaque homologs of Kaposi's sarcoma-associated herpesvirus (KSHV) infect germinal center lymphoid cells, epithelial cells in skin and gastrointestinal tract and gonadal germ cells in naturally infected macaques.
Macaque homologs of Kaposi's sarcoma-associated herpesvirus (KSHV) infect germinal center lymphoid cells, epithelial cells in skin and gastrointestinal tract and gonadal germ cells in naturally infected macaques.
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DOI：
10.1016/j.virol.2018.04.007
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发表时间：
2018-06
期刊：
影响因子：
3.7
通讯作者：
Rose TM
中科院分区：
文献类型：
--
作者：
Bielefeldt-Ohmann H;Bruce AG;Howard K;Ikoma M;Thouless ME;Rose TM
我们开发了一套兔抗血清来表征KSHV的猕猴RV2鼻状病毒同源物的感染。我们分析了恒河猴和猪尾猕猴自然感染恒河猴rhadinovirus（RRV）或猕猴nemestrina rhadinovirus 2（MneRV2）的组织。我们的研究表明，RV2的rhadinoviruses有嗜上皮细胞，淋巴细胞和性腺生殖细胞在体内。我们在未分化和分化的上皮细胞中观察到潜伏感染，表达潜伏标记物拉娜。在口腔、肾脏、皮肤和胃粘膜组织中的上皮导管中的高度分化细胞以及雄性和雌性性腺中的分化生殖细胞中检测到早期（ORF 59）和晚期（糖蛋白B）裂解标记物的表达。我们的数据提供的证据表明，上皮细胞和生殖细胞分化在体内诱导rhadinovirus再激活，并表明，感染的上皮细胞和生殖细胞在体内RV2 rhadinovirus感染的传播和传播中发挥作用。
We developed a set of rabbit antisera to characterize infections by the macaque RV2 rhadinovirus homologs of KSHV. We analyzed tissues from rhesus and pig-tailed macaques naturally infected with rhesus rhadinovirus (RRV) or Macaca nemestrina rhadinovirus 2 (MneRV2). Our study demonstrates that RV2 rhadinoviruses have a tropism for epithelial cells, lymphocytes and gonadal germ cells in vivo. We observed latent infections in both undifferentiated and differentiated epithelial cells with expression of the latency marker, LANA. Expression of the early (ORF59) and late (glycoprotein B) lytic markers were detected in highly differentiated cells in epithelial ducts in oral, renal, dermal and gastric mucosal tissue as well as differentiated germ cells in male and female gonads. Our data provides evidence that epithelial and germ cell differentiation in vivo induces rhadinovirus reactivation and suggests that infected epithelial and germ cells play a role in transmission and dissemination of RV2 rhadinovirus infections in vivo.
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影响因子：
6.7
作者：
Bruce AG;Bielefeldt-Ohmann H;Barcy S;Bakke AM;Lewis P;Tsai CC;Murnane RD;Rose TM
通讯作者：
Rose TM
影响因子：
2.4
作者：
Bielefeldt-Ohmann, H;Barouch, DH;Rose, TM
通讯作者：
Rose, TM
影响因子：
4.8
作者：
Bruce, A. Gregory;Bakke, Angela M.;Rose, Timothy M.
通讯作者：
Rose, Timothy M.
影响因子：
3.7
作者：
Chen, YL;Ciustea, M;Ricciardi, RP
通讯作者：
Ricciardi, RP
影响因子：
5.4
作者：
DeWire, SM;Damania, B
通讯作者：
Damania, B