Construction and characterization of a site-directed CC-1065-N3-adenine adduct within a 117 base pair DNA restriction fragment.
Construction and characterization of a site-directed CC-1065-N3-adenine adduct within a 117 base pair DNA restriction fragment.
复制标题
117 碱基对 DNA 限制性片段内定点 CC-1065-N3-腺嘌呤加合物的构建和表征。
DOI:
10.1021/bi00374a016
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发表时间:
1986
期刊:
影响因子:
2.9
通讯作者:
Hurley,LH
中科院分区:
文献类型:
--
作者:
Needham-VanDevanter,DR;Hurley,LH
Materials and MethodsMaterials CC-1065 was obtained from the Upjohn Co., Kalamazoo, MI. Electrophoretic reagents [acrylamide, TEMED, ammo-nium persulfate, and bis (acrylamide)] were from Bio-Rad. BAP, dNTPs, and lysozyme were from Sigma. All other enzymes [restriction endonucleases, T4-PNK, and DNA polymerase I (large fragment)] were from New EnglandBiolabs. M13mpl vector was obtained from Professor J. Messing, University of Minnesota. MPE was a generous gift of Pro-fessor P. Dervan, California Institute of Technology. M13mpl RF DNA was isolated from infected Escherichia coli JM101 cells by CsCl2 ultracentrifugation as described by Messing (1983).[t-32P] ATP was from ICN. X-ray film, intensifying screen, and developing chemicals were from Kodak.
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DOI:
10.1073/pnas.82.5.1376
发表时间:
1985-01-01
影响因子:
11.1
作者:
KOPKA, ML;YOON, C;DICKERSON, RE
通讯作者:
DICKERSON, RE
影响因子:
2.9
作者:
PETRUSEK, RL;ANDERSON, GL;HURLEY, LH
通讯作者:
HURLEY, LH
影响因子:
2.9
作者:
Johnson,DL;Reid,TM;Lee,MS;King,CM;Romano,LJ
通讯作者:
Romano,LJ
影响因子:
2.9
作者:
Graves,DE;Stone,MP;Krugh,TR
通讯作者:
Krugh,TR
影响因子:
2.9
作者:
Jacobson,MK;Twehous,D;Hurley,LH
通讯作者:
Hurley,LH