Expression of peat moss VASCULAR RELATED NAC-DOMAIN homologs in Nicotiana benthamiana leaf cells induces ectopic secondary wall formation
Expression of peat moss VASCULAR RELATED NAC-DOMAIN homologs in Nicotiana benthamiana leaf cells induces ectopic secondary wall formation
复制标题
泥炭藓血管相关 NAC 域同源物在本塞姆氏烟草叶细胞中的表达诱导异位次生壁形成
DOI:
10.1007/s11103-021-01148-6
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发表时间:
2021
影响因子:
5.1
通讯作者:
Demura Taku
中科院分区:
文献类型:
--
作者:
Terada Shiori;Kubo Minoru;Akiyoshi Nobuhiro;Sano Ryosuke;Nomura Toshihisa;Sawa Shinichiro;Ohtani Misato;Demura Taku
Key messageThe homologs of VASCULAR RELATED NAC-DOMAIN in the peat mossSphagnum palustrewere identified and these transcriptional activity as the VNS family was conserved.AbstractIn angiosperms, xylem vessel element differentiation is governed by the master regulatorsVASCULAR RELATED NAC-DOMAIN6(VND6) andVND7, encoding plant-specific NAC transcription factors. Although vessel elements have not been found in bryophytes, differentiation of the water-conducting hydroid cells in the mossPhyscomitrella patensis regulated byVNDhomologs termedVND-NST-SOMBRERO(VNS) genes.VNSgenes are conserved in the land plant lineage, but their functions have not been elucidated outside of angiosperms andP. patens. The peat mossSphagnum palustre, of class Sphagnopsida in the phylum Bryophyta, does not have hydroids and instead uses hyaline cells with thickened, helical-patterned cell walls and pores to store water in the leaves. Here, we performed whole-transcriptome analysis and de novo assembly using next generation sequencing inS. palustre, obtaining sequences for 68,305 genes. Among them, we identified seven VNS-like genes,SpVNS1-A,SpVNS1-B,SpVNS2-A,SpVNS2-B,SpVNS3-A,SpVNS3-B, andSpVNS4-A. Transient expression of theseVNS-likegenes, with the exception ofSpVNS2-A, inNicotiana benthamianaleaf cells resulted in ectopic thickening of secondary walls. This result suggests that the transcriptional activity observed in other VNS family members is functionally conserved in the VNS homologs ofS. palustre.
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影响因子:
10.5
作者:
Kubo, M;Udagawa, M;Demura, T
通讯作者:
Demura, T
影响因子:
1.1
作者:
Celeste L. Kremer;A. Drinnan
通讯作者:
A. Drinnan
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
Keiko Kobayashi;Masashi Suzuki; Jianwei Tang;Noriko Nagata;Hikaru Seki;Kiyoshi Ohyama;Reiko Kiuchi;Yasuko Kaneko;Shogo Matsumoto;Shigeo Yoshida;Toshiya Muranaka;Masatoshi Yamaguchi
通讯作者:
Masatoshi Yamaguchi
DOI:
--
发表时间:
1998
期刊:
影响因子:
--
作者:
Eva M. Temsch;J. Greilhuber;R. Krisai
通讯作者:
R. Krisai
影响因子:
5.6
作者:
ALTSCHUL, SF;GISH, W;LIPMAN, DJ
通讯作者:
LIPMAN, DJ