The Aborted Microspores (AMS)-Like Gene Is Required for Anther and Microspore Development in Pepper (Capsicum annuum L.).
The Aborted Microspores (AMS)-Like Gene Is Required for Anther and Microspore Development in Pepper (Capsicum annuum L.).
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DOI：
10.3390/ijms19051341
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发表时间：
2018-05-02
影响因子：
5.6
通讯作者：
Shen H
中科院分区：
文献类型：
--
作者：
Guo J;Liu C;Wang P;Cheng Q;Sun L;Yang W;Shen H
辣椒（Capsicum annuum L.）是世界上经济上重要的蔬菜作物。虽然许多与花药和花粉发育相关的基因已经被鉴定出来，但对辣椒花粉败育的机制知之甚少。在这里，我们确定并分离出两个假定的败育小孢子（AMS）异构体从辣椒花：CaAMS 1和CaAMS 2。序列分析表明，CaAMS 2是通过保留CaAMS 1前体mRNA的第四内含子而产生的。CaAMS 1编码一个假定的蛋白质，具有碱性螺旋-环-螺旋（bHLH）结构域，属于bHLH转录因子的MYC亚家族，并且其定位于细胞核。截短的CaAMS 2 -1和CaAMS 2 -2通过选择性剪接产生。实时荧光定量PCR分析表明，CaAMS（简称CaAMS 1和CaAMS 2 -2）在雄蕊中优先表达，随着花的发育，其表达量逐渐降低。RNA原位杂交分析表明，CaAMS在四分体和单核期绒毡层中有强表达。CaAMS的下调导致辣椒花中花丝部分缩短，雄蕊皱缩，不开裂，花粉败育。几个基因参与花粉外壁形成下调有缺陷的CaAMS沉默花药。因此，CaAMS可能通过调控一个复杂的遗传网络在辣椒绒毡层和花粉发育中发挥重要作用。
Pepper (Capsicum annuum L.) is an economically important vegetable crop worldwide. Although many genes associated with anther and pollen development have been identified, little is known about the mechanism of pollen abortion in pepper. Here, we identified and isolated two putative aborted microspore (AMS) isoforms from pepper flowers: CaAMS1 and CaAMS2. Sequence analysis showed that CaAMS2 was generated by retention of the fourth intron in CaAMS1 pre-mRNA. CaAMS1 encodes a putative protein with a basic helix-loop-helix (bHLH) domain belonging to the MYC subfamily of bHLH transcription factors, and it is localized to the nucleus. Truncated CaAMS2-1 and CaAMS2-2 are produced by alternative splicing. Quantitative real-time PCR analysis showed that CaAMS (referred to CaAMS1 and CaAMS2-2) was preferentially expressed in stamens and its expression level gradually decreases with flower development. RNA in situ hybridization analysis showed that CaAMS is strongly expressed in the tapetum at the tetrad and uninucleate stages. Downregulation of CaAMS led to partial shortened filaments, shriveled, indehiscent stamens and abortive pollens in pepper flowers. Several genes involved in pollen exine formation were downregulated in defective CaAMS-silenced anthers. Thus, CaAMS seems to play an important role in pepper tapetum and pollen development by regulating a complex genetic network.
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影响因子：
7.4
作者：
Dobritsa, Anna A.;Lei, Zhentian;Sumner, Lloyd W.
通讯作者：
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影响因子：
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64.5
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