A comparison of manual neuronal reconstruction from biocytin histology or 2-photon imaging: morphometry and computer modeling.
A comparison of manual neuronal reconstruction from biocytin histology or 2-photon imaging: morphometry and computer modeling.
复制标题
DOI：
10.3389/fnana.2014.00065
复制
发表时间：
2014
影响因子：
2.9
通讯作者：
Sjöström PJ
中科院分区：
文献类型：
--
作者：
Blackman AV;Grabuschnig S;Legenstein R;Sjöström PJ
神经元的精确三维重建对于连接解剖学和生理学的应用至关重要。重建通常是在生物细胞素组织学（BH）后使用Neurolucida创建的。另一种廉价而快速的方法是使用免费软件，如Neuromantic，从生理记录期间使用双光子激光扫描显微镜获得的荧光成像（FI）堆栈进行重建。我们比较了这两种方法在形态学、细胞分类和神经元模拟环境中的多室建模。使用这两种方法重建的相同细胞的定量形态学分析表明，虽然生物细胞素重建有助于追踪更多的远侧侧枝，但这两种方法在代表整体形态学方面具有可比性：两种方法重建的自动聚类成功地将新皮质篮状细胞从锥体细胞中分离出来，但不能将BH从FI重建中分离出来。BH重建比FI重建更容易受到组织收缩和压缩伪影的影响。另一方面，FI重建始终具有更大的工艺直径。因此，两种方法在兴奋性突触后电位（EPSP）前向传播的神经元建模上存在显著差异，FI重建显示出较小的去极化。然而，模拟动作电位反向传播（bAP）在两种方法获得的重建结果之间是无法区分的。在我们手中，BH重建对于神经元建模和详细的形态追踪是必要的，因此仍然是最先进的，尽管它们更劳动密集，更昂贵，并且由于组织学处理的偶尔不良结果而遭受更高的故障率。然而，对于细胞类型鉴定等解剖学应用的一个子集，FI重建是优越的，因为它具有不可区分的分类性能，更易于使用，基本上100%的成功率和更低的成本。
Accurate 3D reconstruction of neurons is vital for applications linking anatomy and physiology. Reconstructions are typically created using Neurolucida after biocytin histology (BH). An alternative inexpensive and fast method is to use freeware such as Neuromantic to reconstruct from fluorescence imaging (FI) stacks acquired using 2-photon laser-scanning microscopy during physiological recording. We compare these two methods with respect to morphometry, cell classification, and multicompartmental modeling in the NEURON simulation environment. Quantitative morphological analysis of the same cells reconstructed using both methods reveals that whilst biocytin reconstructions facilitate tracing of more distal collaterals, both methods are comparable in representing the overall morphology: automated clustering of reconstructions from both methods successfully separates neocortical basket cells from pyramidal cells but not BH from FI reconstructions. BH reconstructions suffer more from tissue shrinkage and compression artifacts than FI reconstructions do. FI reconstructions, on the other hand, consistently have larger process diameters. Consequently, significant differences in NEURON modeling of excitatory post-synaptic potential (EPSP) forward propagation are seen between the two methods, with FI reconstructions exhibiting smaller depolarizations. Simulated action potential backpropagation (bAP), however, is indistinguishable between reconstructions obtained with the two methods. In our hands, BH reconstructions are necessary for NEURON modeling and detailed morphological tracing, and thus remain state of the art, although they are more labor intensive, more expensive, and suffer from a higher failure rate due to the occasional poor outcome of histological processing. However, for a subset of anatomical applications such as cell type identification, FI reconstructions are superior, because of indistinguishable classification performance with greater ease of use, essentially 100% success rate, and lower cost.
登录
查看更多内容
影响因子：
2.5
作者：
Losavio, Bradley E.;Liang, Yong;Saggau, Peter
通讯作者：
Saggau, Peter
影响因子：
--
作者：
Markram, Henry
通讯作者：
Markram, Henry
影响因子：
16.2
作者：
Gidon, Albert;Segev, Idan
通讯作者：
Segev, Idan
影响因子：
16.2
作者：
Ding, Jun B.;Takasaki, Kevin T.;Sabatini, Bernardo L.
通讯作者：
Sabatini, Bernardo L.
影响因子：
34.7
作者：
Ascoli, Giorgio A.;Alonso-Nanclares, Lidia;Anderson, Stewart A.;Barrionuevo, German;Benavides-Piccione, Ruth;Burkhalter, Andreas;Buzsaki, Gyoergy;Cauli, Bruno;DeFelipe, Javier;Fairen, Alfonso;Feldmeyer, Dirk;Fishell, Gord;Fregnac, Yves;Freund, Tamas F.;Gardner, Daniel;Gardner, Esther P.;Goldberg, Jesse H.;Helmstaedter, Moritz;Hestrin, Shaul;Karube, Fuyuki;Kisvarday, Zoltan F.;Lambolez, Bertrand;Lewis, David A.;Marin, Oscar;Markram, Henry;Munoz, Alberto;Packer, Adam;Petersen, Carl C. H.;Rockland, Kathleen S.;Rossier, Jean;Rudy, Bernardo;Somogyi, Peter;Staiger, Jochen F.;Tamas, Gabor;Thomson, Alex M.;Toledo-Rodriguez, Maria;Wang, Yun;West, David C.;Yuste, Rafael
通讯作者：
Yuste, Rafael