Effect of Morinda citrifolia (Noni)-Enriched Diet on Hepatic Heat Shock Protein and Lipid Metabolism-Related Genes in Heat Stressed Broiler Chickens.
Effect of Morinda citrifolia (Noni)-Enriched Diet on Hepatic Heat Shock Protein and Lipid Metabolism-Related Genes in Heat Stressed Broiler Chickens.
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巴戟天强化日粮对热应激肉鸡肝脏热休克蛋白和脂代谢相关基因的影响
DOI：
10.3389/fphys.2017.00919
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发表时间：
2017
影响因子：
4
通讯作者：
Dridi S
中科院分区：
文献类型：
--
作者：
Flees J;Rajaei-Sharifabadi H;Greene E;Beer L;Hargis BM;Ellestad L;Porter T;Donoghue A;Bottje WG;Dridi S
据报道，热应激（HS）可改变肉鸡脂肪沉积，但其潜在的分子机制尚不明确。因此，本研究的目的是：(1)确定急性（2小时）和慢性（3周）HS对肝脏脂肪生成和脂肪分解程序中涉及的关键分子特征表达的影响，(2)评估添加干诺丽药用植物（饮食的0.2%）是否调节这些影响。试验采用2 × 2因子设计，将480只雄性1 d肉鸡随机分配到12个环境舱中，分别进行2种环境条件（热应激，HS， 35°C vs热中性条件，TN, 24°C）和2种饲粮（对照vs诺丽）试验。分别于热暴露后2 h和3周记录采食量和体重，采集血液和肝脏样本。HS降低采食量，降低体重，上调肝脏热休克蛋白HSP60、HSP70、HSP90及关键脂肪生成蛋白（脂肪酸合成酶FASN、乙酰辅酶a羧化酶α、ACCα和ATP柠檬酸裂解酶ACLY）的表达。HS下调肝脏脂蛋白脂肪酶（LPL）和肝甘油三酯脂肪酶（LIPC）的表达，上调ATGL的表达。虽然它不影响生长性能，但诺丽补充剂以时间和基因特异性的方式调节肝脏脂肪生成蛋白的表达。在HS之前，Noni在急性和慢性实验条件下分别增加ACLY和FASN。急性HS时，Noni增加了ACCα，但降低了FASN和ACLY的表达。慢性HS下，Noni上调ACCα和FASN，下调ACLY。体外对鸡肝细胞系的研究表明，HS可下调ACCα、FASN和ACLY的表达。槲皮素是诺丽草中的一种生物活性成分，在TN条件下，槲皮素上调了ACCα、FASN和ACLY的表达，但在HS条件下，槲皮素似乎下调了ACCα，增加了ACLY的表达。总之，我们的研究结果表明，HS诱导鸡肝脏脂肪生成，这种作用可能是通过HSPs介导的。对肝脏热休克蛋白表达的调节也提示诺丽可能参与了鸡肝脏应激反应的调节。
Heat stress (HS) has been reported to alter fat deposition in broilers, however the underlying molecular mechanisms are not well-defined. The objectives of the current study were, therefore: (1) to determine the effects of acute (2 h) and chronic (3 weeks) HS on the expression of key molecular signatures involved in hepatic lipogenic and lipolytic programs, and (2) to assess if diet supplementation with dried Noni medicinal plant (0.2% of the diet) modulates these effects. Broilers (480 males, 1 d) were randomly assigned to 12 environmental chambers, subjected to two environmental conditions (heat stress, HS, 35°C vs. thermoneutral condition, TN, 24°C) and fed two diets (control vs. Noni) in a 2 × 2 factorial design. Feed intake and body weights were recorded, and blood and liver samples were collected at 2 h and 3 weeks post-heat exposure. HS depressed feed intake, reduced body weight, and up regulated the hepatic expression of heat shock protein HSP60, HSP70, HSP90 as well as key lipogenic proteins (fatty acid synthase, FASN; acetyl co-A carboxylase alpha, ACCα and ATP citrate lyase, ACLY). HS down regulated the hepatic expression of lipoprotein lipase (LPL) and hepatic triacylglycerol lipase (LIPC), but up-regulated ATGL. Although it did not affect growth performance, Noni supplementation regulated the hepatic expression of lipogenic proteins in a time- and gene-specific manner. Prior to HS, Noni increased ACLY and FASN in the acute and chronic experimental conditions, respectively. During acute HS, Noni increased ACCα, but reduced FASN and ACLY expression. Under chronic HS, Noni up regulated ACCα and FASN but it down regulated ACLY. In vitro studies, using chicken hepatocyte cell lines, showed that HS down-regulated the expression of ACCα, FASN, and ACLY. Treatment with quercetin, one bioactive ingredient in Noni, up-regulated the expression of ACCα, FASN, and ACLY under TN conditions, but it appeared to down-regulate ACCα and increase ACLY levels under HS exposure. In conclusion, our findings indicate that HS induces hepatic lipogenesis in chickens and this effect is probably mediated via HSPs. The modulation of hepatic HSP expression suggest also that Noni might be involved in modulating the stress response in chicken liver.
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影响因子：
3.8
作者：
Bhusari, Sachin;Liu, Zhilin;Antoniou, Eric
通讯作者：
Antoniou, Eric
影响因子：
3.9
作者：
Brownsey, RW;Boone, AN;Lee, WM
通讯作者：
Lee, WM
影响因子：
4.2
作者：
Hermier, D
通讯作者：
Hermier, D
影响因子：
4.4
作者：
Deeb, N;Cahaner, A
通讯作者：
Cahaner, A
影响因子：
4.8
作者：
Goti, D;Balazs, Z;Sattler, W
通讯作者：
Sattler, W