The contractile action of platelet-activating factor on gallbladder smooth muscle.
The contractile action of platelet-activating factor on gallbladder smooth muscle.
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血小板活化因子对胆囊平滑肌的收缩作用。
DOI：
10.1152/ajpgi.2000.279.1.g67
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Ryan,JP
中科院分区：
文献类型：
--
作者：
Parkman,HP;James,AN;Ryan,JP
血小板活化因子（PAF）可能是胆囊炎（一种胆囊运动功能障碍的疾病）某些后遗症的介导因素。本研究旨在探讨PAF对胆囊肌的作用及其机制。PAF-16和PAF-18可引起胆囊肌条收缩，其阈值剂量分别为1 ng/ml和10 ng/ml。PAF诱导的收缩未被TTX、阿托品或六甲双铵显著降低，但被PAF受体拮抗剂银杏叶提取物B和CV-3988显著抑制。PAF引起的收缩被消炎痛减弱。用无钙溶液阻止细胞外钙内流几乎消除了PAF的收缩反应。硝苯地平抑制PAF的收缩反应，而ryanodine没有影响。百日咳毒素降低PAF的收缩反应。结论：PAF通过胆囊肌上特异性的PAF受体引起胆囊收缩。这些PAF受体似乎与白藜芦醇苷介导的机制和百日咳毒素敏感的G蛋白有关。收缩反应主要是通过利用细胞外钙内流通过电压依赖性钙通道介导的。
Platelet-activating factor (PAF) may be a mediator of some sequelae of cholecystitis, a disorder with gallbladder motor dysfunction. The aims of this study were to determine the effect and mechanism of PAF on gallbladder muscle. Exogenous administration of PAF-16 or PAF-18 caused dose-dependent contractions of gallbladder muscle strips in vitro with threshold doses of 1 ng/ml and 10 ng/ml, respectively. The PAF-induced contractions were not significantly reduced by TTX, atropine, or hexamethonium but were significantly inhibited with the PAF receptor antagonists ginkolide B and CV-3988. The PAF-induced contraction was reduced by indomethacin. Preventing influx of extracellular calcium with a calcium-free solution nearly abolished the PAF contractile response. Nifedipine inhibited the PAF contractile response, whereas ryanodine had no effect. Pertussis toxin reduced the PAF contractile response. In conclusion, PAF causes gallbladder contraction through specific PAF receptors on gallbladder muscle. These PAF receptors appear to be linked to a prostaglandin-mediated mechanism and to pertussis toxin-sensitive G proteins. The contractile response is largely mediated through the utilization of extracellular calcium influx through voltage-dependent calcium channels.
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影响因子：
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作者：
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DOI：
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期刊：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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DOI：
--
发表时间：
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期刊：
The Journal of pharmacology and experimental therapeutics.
影响因子：
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作者：
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通讯作者：
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