Antioxidant defence system of boar cauda epididymidal spermatozoa and reproductive tract fluids.
Antioxidant defence system of boar cauda epididymidal spermatozoa and reproductive tract fluids.
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公猪尾部附睾精子和生殖道液体的抗氧化防御系统。
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
J. Strzeżek
中科院分区：
文献类型：
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作者：
M. Koziorowska;M. Koziorowski;Leyland Fraser;J. Strzeżek
生殖道分泌的抗氧化剂可以保护精子免受射精后活性氧（ROS）的毒性作用。本研究旨在表征公猪附睾尾部精子以及附睾尾部、囊泡和前列腺液的抗氧化保护水平。此外，本研究还研究了 5 小时透析对公猪精浆抗氧化能力的影响。监测附睾精子尾部或生殖道液体中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）、谷胱甘肽转移酶（GST）和磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPx）活性。此外，还测量了液体中总谷胱甘肽 (GSH + GSSG)、L-麦角硫因 (ERT) 和 L-抗坏血酸的浓度以及总抗氧化状态 (TAS)。结果发现附睾精子尾部表现出较高的SOD活性和相对较低的PHGPx活性。附睾精子尾部的 GPx、GR 和 GST 活性的相对量可以忽略不计，而 CAT 活性则检测不到。在附睾尾和前列腺的液体中发现了更高的 SOD 活性。此外，前列腺液似乎是精浆中CAT活性的主要来源，而最高水平的GPx活性来自附睾尾液。生殖道液体中的 GR 和 GST 活性可忽略不计。生殖道液中大量的 GSH + GSSG、ERT 和 L-抗坏血酸似乎可以对精浆中的 TAS 水平具有改善作用。透析对精浆总抗氧化能力有显着影响，表现为SOD和GPx活性增强。这项研究的结果证实，精浆的清除潜力取决于不同抗氧化剂的贡献，这些抗氧化剂源自公猪生殖道的各种液体。
Antioxidants secreted by the reproductive tract protect spermatozoa against the toxic effects of reactive oxygen species (ROS) after ejaculation. This study aimed at characterizing the level of antioxidant protection in boar cauda epididymidal spermatozoa and fluids of the cauda epididymidis, vesicular and prostate glands. Also, this study investigated the effect of a 5-h period of dialysis on the antioxidant capacity of boar seminal plasma. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione transferase (GST) and phospholipid hydroperoxide glutathione peroxidase (PHGPx) activities were monitored in the cauda epididymidal spermatozoa or reproductive tract fluids. Also, the concentrations of total glutathione (GSH + GSSG), L-ergothioneine (ERT) and l-ascorbate and the total antioxidant status (TAS) of the fluids were measured. It was found that the cauda epididymidal spermatozoa exhibited high SOD activity and relatively low activity of PHGPx. The relative amounts of GPx, GR and GST activities in the cauda epididymidal spermatozoa were negligible, whereas CAT activity was undetectable. Greater SOD activity was found in the fluids of the cauda epididymidis and prostate gland. Furthermore, the prostate gland fluid appeared to be the main source of CAT activity in the seminal plasma, whereas the highest level of GPx activity was derived from the cauda epididymidal fluid. The reproductive tract fluids exhibited negligible amounts of GR and GST activities. It seemed that the significant amounts of GSH + GSSG, ERT and L-ascorbate in the reproductive tract fluids could have an ameliorative effect on the level of TAS in the seminal plasma. Dialysis had a marked effect on the total antioxidant capacity of the seminal plasma, which was manifested in greater activity of SOD and GPx. The findings of this study confirmed that the scavenging potential of the seminal plasma is dependent on the contributions of different antioxidants, originating in various fluids of boar reproductive tract.
影响因子：
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作者：
J. Alvarez;J. Touchstone;L. Blasco;B. Storey
通讯作者：
J. Alvarez;J. Touchstone;L. Blasco;B. Storey