Analysis of MHC class I genes across horse MHC haplotypes.
Analysis of MHC class I genes across horse MHC haplotypes.
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DOI：
10.1007/s00251-009-0420-9
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发表时间：
2010-03
期刊：
影响因子：
3.2
通讯作者：
Antczak DF
中科院分区：
文献类型：
--
作者：
Tallmadge RL;Campbell JA;Miller DC;Antczak DF
利用15个马主要组织相容性复合体(MHC)I类基因的基因组序列和5个不同单倍型的MHC I类纯合子马，研究了马MHC的基因组结构和多态性。采用保守性和位点特异性相结合的方法，扩增出具有经典和非经典特征的马MHC I类基因。来自每个单倍型的多个克隆确定了每个纯合子动物三到五个经典序列，以及两到三个非经典序列。对这些序列进行了系统发育分析，鉴定出了看似等位基因序列的类群，但有一些序列没有归类。然后添加从MHC I类杂合马确定的序列和先前描述的MHC I类序列，代表总共10个马MHC单倍型。这些结果与仅从MHC纯合子马获得的结果一致，并将30个经典序列归类为4个先前确认的座位和3个新的临时座位。非经典基因等位基因较少，经典基因具有较高的等位基因多态水平。在测试的大多数单倍型中，发现了两个具有预期多态模式的经典基因座的等位基因，但另外两个常见的基因座的等位基因在高变区之外的变异比在高变区内的变异更大。我们的数据表明，马的主要组织相容性复合体的特征是在不同单倍型中表达的I类基因的补体存在差异，以及预期的基因座内等位基因多态。
The genomic sequences of 15 horse Major Histocompatibility Complex (MHC) class I genes and a collection of MHC class I homozygous horses of five different haplotypes were used to investigate the genomic structure and polymorphism of the equine MHC. A combination of conserved and locus-specific primers was used to amplify horse MHC class I genes with classical and non-classical characteristics. Multiple clones from each haplotype identified three to five classical sequences per homozygous animal, and two to three non-classical sequences. Phylogenetic analysis was applied to these sequences and groups were identified which appear to be allelic series, but some sequences were left ungrouped. Sequences determined from MHC class I heterozygous horses and previously described MHC class I sequences were then added, representing a total of ten horse MHC haplotypes. These results were consistent with those obtained from the MHC homozygous horses alone, and 30 classical sequences were assigned to four previously confirmed loci and three new provisional loci. The non-classical genes had few alleles and the classical genes had higher levels of allelic polymorphism. Alleles for two classical loci with the expected pattern of polymorphism were found in the majority of haplotypes tested, but alleles at two other commonly detected loci had more variation outside of the hypervariable region than within. Our data indicate that the equine Major Histocompatibility Complex is characterized by variation in the complement of class I genes expressed in different haplotypes in addition to the expected allelic polymorphism within loci.
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影响因子：
2.7
作者：
Johnson, DR
通讯作者：
Johnson, DR
影响因子：
10.7
作者：
Gu, X;Nei, M
通讯作者：
Nei, M
影响因子：
3.2
作者：
Ellis, SA;Holmes, EC;Morrison, WI
通讯作者：
Morrison, WI
DOI：
10.1111/j.1744-313x.1995.tb00239.x
发表时间：
1995-06-01
期刊：
EUROPEAN JOURNAL OF IMMUNOGENETICS
影响因子：
--
作者：
ELLIS, SA;MARTIN, AJ;MORRISON, WI
通讯作者：
MORRISON, WI
影响因子：
9.5
作者：
Kumar, S;Tamura, K;Nei, M
通讯作者：
Nei, M