Selection of T cells reactive against autologous B lymphoblastoid cells during chronic rheumatoid arthritis.
Selection of T cells reactive against autologous B lymphoblastoid cells during chronic rheumatoid arthritis.
复制标题
慢性类风湿关节炎期间对自体 B 淋巴母细胞有反应的 T 细胞的选择。
作者：
J. David;A. Lim;F. Davodeau;M. Peyrat;J. Berthelot;G. Semana;C. Pannetier;J. Gaschet;H. Vié;J. Even;M. Bonneville
对1例慢性类风湿关节炎(RA)患者炎性关节T细胞的免疫表型和抗原特异性进行了详细研究。系谱分析显示，与患者的PBL相比，关节浸润性淋巴细胞(JIL)的克隆性降低，这可能是由于具有复发TCR特征的T细胞克隆在关节内扩张所致。值得注意的是，与随机选择的来自同一患者的PBL克隆不同，这些以CD8+为主的JIL T细胞克隆在暴露于自体B淋巴母细胞(BLC)的情况下，在体外增殖。这种增殖反应是受HLA限制的，这证实了经典的TCR介导的对BLC的识别，并且没有观察到对自体PHA母细胞的识别，这表明可以识别EBV或B细胞特异性的AGS。最后，来自另一位慢性RA患者的滑膜淋巴细胞的初步分析显示，与患者的PBL相比，JIL中对自体BLC反应的T细胞具有类似的浓缩作用。综上所述，这些结果提示慢性RA患者炎症关节内自体BLC反应性T细胞频繁扩张，为未来评估这些淋巴细胞的良好特异性和致病性提供了基础。
The repertoire and Ag specificity of T cells infiltrating inflamed joints from a chronic rheumatoid arthritis (RA) patient were studied in detail. Repertoire analysis demonstrated a reduced clonality of joint-infiltrating lymphocytes (JIL) as compared with patient's PBL, which was presumably due to an intra-articular expansion of T cell clones with recurrent TCR features. Strikingly, a large fraction of these JIL T cell clones, which were predominantly CD8+, proliferated in vitro when exposed to autologous B lymphoblastoid cells (BLC), unlike randomly chosen PBL clones derived from the same patient. This proliferative response was HLA-restricted, which confirmed a classical TCR-mediated recognition of BLC and was not observed against autologous PHA blasts, suggesting recognition of either EBV or B cell-specific Ags. Finally, a preliminary analysis of synovial lymphocytes derived from another chronic RA patient demonstrated a similar enrichment for T cells reactive against autologous BLC within JILs as compared with patient's PBLs. Taken together, these results, which suggest frequent expansions of autologous BLC-reactive T cells within inflamed joints of chronic RA patients, provide a basis for future studies evaluating the fine specificity and pathogenicity of these lymphocytes.
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影响因子：
56.9
作者：
PALIARD, X;WEST, SG;KOTZIN, BL
通讯作者：
KOTZIN, BL
寡克隆类风湿关节炎滑膜 T 细胞共有 CDR3 序列基序。
DOI：
10.1172/jci117624
发表时间：
1994
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Li,Y;Sun,GR;Tumang,JR;Crow,MK;Friedman,SM
通讯作者：
Friedman,SM
类风湿性关节炎患者中 Vβ17 阳性 T 细胞的频率增加。
影响因子：
--
作者：
Zagon,G;Tumang,JR;Li,Y;Friedman,SM;Crow,MK
通讯作者：
Crow,MK
正常人类受试者中 CD8 CD45RO 亚群中 T 细胞受体的克隆优势。
DOI：
--
发表时间：
1993
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hingorani,R;Choi,IH;Akolkar,P;Gulwani-Akolkar,B;Pergolizzi,R;Silver,J;Gregersen,PK
通讯作者：
Gregersen,PK