Multiple translation initiation factor Sui1 related sequences in mammalian genomes
Multiple translation initiation factor Sui1 related sequences in mammalian genomes
复制标题
哺乳动物基因组中多个翻译起始因子Sui1相关序列
作者：
R. Purohit;D. McCormick;J. Dyson
当通过逆转录酶聚合酶链反应（RT PCR）方法从DBA/2 T细胞克隆中克隆小鼠T细胞受体（TCR）cDNA时（Casanova等，1991），我们发现分离的一个克隆是Suil基因的部分（355 bp）小鼠同源物。Suil首先在设计用于分离参与起始密码子的核糖体识别的基因的遗传筛选中从酿酒酵母中分离（Castilho-Valavicius等，1990）。该基因编码一种高度保守的新型翻译起始因子，该因子在翻译起始复合物中与真核起始因子2（eIF2）相互作用，将AUG登记为起始密码子（Yoon and Donahue 1992）。基因破坏实验已经证明Suil基因对于S.酿酒酵母（Yoon和Donahue 1992）。分离的小鼠序列与人SUII共有序列（SUIIISO1; Fields和亚当斯1994）具有超过95%的同源性，并且由于与用于PCR扩增cDNA的TCR寡核苷酸具有中等的序列同源性而分离。由于人类SUII基因的基因组组织尚未被描述，也没有其他哺乳动物的同源物已被确定，我们有兴趣调查在小鼠基因组中的SuII基因组织。在高严格性下用小鼠Suil探针进行的Southern印迹分析鉴定了使用所有限制性内切酶的多个条带;杂交模式的复杂性表明小鼠基因组中存在多个Suil相关序列。为了证实这一解释并进一步确定Suil相关序列是否分散或存在于单个染色体上，我们鉴定了C57 BL/6和DBA/2近交系小鼠品系之间的限制性片段长度多态性（RFLP），以使BXD重组近交系品系（Taylor 1989）能够用于基因作图。多种限制性内切酶（KpnI、SacI、Hpal、EcoRI、BarnHl、Taql和HincII）产生RFLP。图1显示了用限制性内切酶Taql消化的BXD RI系列的一个例子;这说明了小鼠基因组中的杂交复杂性：指出了由该酶鉴定的两种多态性。七个RFLPs可以明确分型在所有的BXD菌株，这些产生了四个菌株分布模式（SDPs）。将这些SDP与BXD数据库进行比较，将Suil相关序列的拷贝定位于小鼠染色体（Chrs）3、5、8和13，而没有重组体定位于先前描述的基因座（表1）。这一分析清楚地表明，Suil家族分散在小鼠基因组的几条染色体上;杂交模式的复杂性意味着这四个映射的成员不太可能代表整个家族。为了确定Suil是否也是其他哺乳动物物种中的序列家族，制备动物园印迹并用小鼠Suil探针探测（图2）。该分析在小鼠、人、兔、负鼠和猪中显示多个杂交条带。因此，Suil被认为是这些哺乳动物物种中的一个序列家族;
While cloning mouse T cell receptor (TCR) cDNAs from DBA/2 T cell clones by a reverse transcriptase polymerase chain reaction (RT PCR) approach (Casanova et al. 1991), we found one clone isolated to be a partial (355 bp) mouse homolog of the Suil gene. Suil was first isolated from Saccharomyces cerevisiae in a genetic screen designed to isolate genes involved in ribosomal recognition of an initiator codon (Castilho-Valavicius et al. 1990). The gene encodes a highly conserved, novel translation initiation factor that interacts with eukaryotic initiation factor 2 (elF2) in the translation initiation complex to register AUG as an initiator codon (Yoon and Donahue 1992). Gene disruption experiments have demonstrated the Suil gene to be essential for cell viability in S. cerevisiae (Yoon and Donahue 1992). The isolated mouse sequence was over 95% homologous to the human SUII consensus sequence (SUIIISO1; Fields and Adams 1994) and was isolated owing to moderate sequence homology with the TCR oligonucleotides used for PCR amplification of cDNA. Since the genomic organization of the human SUII gene has not been described and no other mammalian homolog has been identified, we were interested to investigate Suil gene organization in the mouse genome. Southern blot analysis performed at high stringency with the mouse Suil probe identified multiple bands with all restriction enzymes used; the complexity of the hybridization pattern suggested that multiple Suil-related sequences are present in the mouse genome. To confirm this interpretation and to further define whether the Suil-related sequences are dispersed or reside on a single chromosome, we identified Restriction Fragment Length Polymorphisms (RFLPs) between the C57BL/6 and DBA/2 inbred mouse strains to enable the BXD recombinant inbred strains (Taylor 1989) to be used for gene mapping. Multiple restriction endonucleases (Kpnl, SacI, Hpal, EcoRI, BarnHl, Taql, and HincII) yielded RFLPs. An example of the BXD RI series digested with the restriction endonuclease Taql is shown in Fig. 1 ; this illustrates the hybridization complexity in the mouse genome: two polymorphisms identified by this enzyme are indicated. Seven RFLPs could be unambiguously typed in all the BXD strains; these yielded four strain distribution patterns (SDPs). Comparison of these SDPs to the BXD database localized copies of Suil-related sequence to mouse Chromosomes (Chrs) 3, 5, 8, and 13 with no recombinants to previously described loci (Table 1). This analysis clearly demonstrates the Suil family to be dispersed on several chromosomes in the mouse genome; the complexity of the hybridization pattern implies that the four mapped members are unlikely to represent the entire family. To ascertain whether Suil is also a sequence family in other mammalian species, a zoo blot was prepared and probed with the mouse Suil probe (Fig. 2). This analysis revealed multiple hybridizing bands in mouse, human, rabbit, opossum, and pig. Suil is thus interred to be a sequence family in these mammalian species;
酿酒酵母翻译起始抑制子 sui1、sui2 和 SUI3 的遗传特征及其对 HIS4 表达的影响。
影响因子：
3.3
作者：
Castilho-Valavicius,B;Yoon,H;Donahue,TF
通讯作者：
Donahue,TF
适合进行种内杂交分型的小鼠遗传图谱。
影响因子：
3.3
作者：
Dietrich,W;Katz,H;Lincoln,SE;Shin,HS;Friedman,J;Dracopoli,NC;Lander,ES
通讯作者：
Lander,ES