Optogenetic regulation of transcription.
Optogenetic regulation of transcription.
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DOI：
10.1186/s12868-018-0411-6
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发表时间：
2018-04-19
期刊：
影响因子：
2.4
通讯作者：
Myakishev-Rempel M
中科院分区：
文献类型：
--
作者：
Polesskaya O;Baranova A;Bui S;Kondratev N;Kananykhina E;Nazarenko O;Shapiro T;Nardia FB;Kornienko V;Chandhoke V;Stadler I;Lanzafame R;Myakishev-Rempel M
光遗传学已成为广泛认可的成功实时控制脑神经元利用非哺乳动物的光敏蛋白打开或关闭膜通道。在这里，我们回顾了一种不太为人所知的光遗传学结构类型，它采用光敏蛋白来抑制信号以调节基因转录，以及它在医学中的可能用途。现有基因疗法的问题之一是它们可以无限期地保持活性，同时不允许按需调节转基因生产。转录的光遗传调节（ORT）可潜在地用于调节原位生物药物的产生，通过反复地向组织施加光，并在需要时诱导治疗性转基因的表达。红色和近红外波长能够穿透到组织中，具有治疗应用的潜力。在考虑到这些因素的情况下，本文对现有的ORT系统进行了审查。
Optogenetics has become widely recognized for its success in real-time control of brain neurons by utilizing non-mammalian photosensitive proteins to open or close membrane channels. Here we review a less well known type of optogenetic constructs that employs photosensitive proteins to transduce the signal to regulate gene transcription, and its possible use in medicine. One of the problems with existing gene therapies is that they could remain active indefinitely while not allowing regulated transgene production on demand. Optogenetic regulation of transcription (ORT) could potentially be used to regulate the production of a biological drug in situ, by repeatedly applying light to the tissue, and inducing expression of therapeutic transgenes when needed. Red and near infrared wavelengths, which are capable of penetration into tissues, have potential for therapeutic applications. Existing ORT systems are reviewed herein with these considerations in mind.
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影响因子：
64.8
作者：
通讯作者：
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影响因子：
16.6
作者：
Folcher, Marc;Oesterle, Sabine;Zwicky, Katharina;Thekkottil, Thushara;Heymoz, Julie;Hohmann, Muriel;Christen, Matthias;El-Baba, Marie Daoud;Buchmann, Peter;Fussenegger, Martin
通讯作者：
Fussenegger, Martin
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Kennedy, Matthew J.;Hughes, Robert M.;Peteya, Leslie A.;Schwartz, Joel W.;Ehlers, Michael D.;Tucker, Chandra L.
通讯作者：
Tucker, Chandra L.
影响因子：
25
作者：
Lin, John Y.;Knutsen, Per Magne;Muller, Arnaud;Kleinfeld, David;Tsien, Roger Y.
通讯作者：
Tsien, Roger Y.
影响因子：
3.6
作者：
Das AT;Tenenbaum L;Berkhout B
通讯作者：
Berkhout B