Elevated expression of both mRNA and protein levels of IL-17A in sputum of stable Cystic Fibrosis patients.
Elevated expression of both mRNA and protein levels of IL-17A in sputum of stable Cystic Fibrosis patients.
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DOI：
10.1186/1465-9921-11-177
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发表时间：
2010-12-10
影响因子：
5.8
通讯作者：
Dupont LJ
中科院分区：
文献类型：
--
作者：
Decraene A;Willems-Widyastuti A;Kasran A;De Boeck K;Bullens DM;Dupont LJ
辅助性T细胞(Th17)可以通过产生IL-17将中性粒细胞募集到炎症部位，从而诱导趋化因子的释放。IL-23是IL-17和IL-22产生的重要诱导剂。本研究通过检测临床稳定的囊性纤维化患者痰中IL-17蛋白、IL-22、IL-23的表达水平，并与健康对照组比较，探讨Th17细胞在囊性纤维化肺疾病中的作用。成人慢性阻塞性肺疾病患者在病情恶化之外和健康对照组中进行诱导痰。用流式细胞仪检测上清液中IL-17A蛋白水平，提取RNA，定量RT-PCR检测IL-17A、IL-22和IL-23的表达。我们发现与对照组相比，CF组痰中IL-17A蛋白和IL-23mRNA水平(P<；0.0001)和IL-23mRNA水平(P<；0.0001)均显著升高。我们发现在CF组中IL-22的mRNA水平很低。慢性感染铜绿假单胞菌患者IL-17和IL-23mRNA水平高于非慢性感染患者。痰中金黄色葡萄球菌的存在不影响IL-17或IL-23水平。IL-17、IL-23水平与FEV1、痰中性粒细胞均无相关性。IL-17和IL-23水平的升高可能提示Th17细胞参与了慢性肺病和慢性铜绿假单胞菌感染中性粒细胞的持续浸润。
T helper 17 (Th17) cells can recruit neutrophils to inflammatory sites through production of IL-17, which induces chemokine release. IL-23 is an important inducer of IL-17 and IL-22 production. Our aim was to study the role of Th17 cells in cystic fibrosis (CF) lung disease by measuring IL-17 protein and mRNA levels and IL-22 and IL-23 mRNA in sputum of clinically stable CF patients and by comparing these levels with healthy controls. Sputum induction was performed in adult CF patients outside of an exacerbation and healthy control subjects. IL-17A protein levels were measured in supernatants with cytometric bead array (CBA) and RNA was isolated and quantitative RT-PCR was performed for IL-17A, IL-22 and IL-23. We found significantly higher levels of IL-17A protein and mRNA levels (both: p < 0.0001) and IL-23 mRNA levels (p < 0.0001) in the sputum of CF group as compared to controls. We found very low levels of IL-22 mRNA in the CF group. The levels of IL-17 and IL-23 mRNA were higher in patients chronically infected with Pseudomonas aeruginosa (P. aeruginosa) as compared to those who were not chronically infected with P. aeruginosa. The presence of Staphylococcus aureus (S. aureus) on sputum did not affect the IL-17 or IL-23 levels. There was no correlation between IL-17 or IL-23 levels and FEV1 nor sputum neutrophilia. The elevated levels of IL-17 and IL-23 might indicate that Th17 cells are implicated in the persistent neutrophil infiltration in CF lung disease and chronic infection with P. aeruginosa.
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影响因子：
10
作者：
Mackerness, K. J.;Jenkins, G. R.;Jose, P. J.
通讯作者：
Jose, P. J.
影响因子：
24.1
作者：
Peng, Meiyu;Wang, Zhaohua;Li, Baiqing
通讯作者：
Li, Baiqing
影响因子：
168.9
作者：
JOHANSEN, HK;NIR, M;SCHWARTZ, M
通讯作者：
SCHWARTZ, M
影响因子：
14.2
作者：
Molet, S;Hamid, Q;Chakir, J
通讯作者：
Chakir, J
影响因子：
14.2
作者：
Hoshino, H;Laan, M;Lindén, A
通讯作者：
Lindén, A