Utility of transplantation in studying adipocyte biogenesis and function.
Utility of transplantation in studying adipocyte biogenesis and function.
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DOI：
10.1016/j.mce.2009.08.022
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发表时间：
2010-04-29
影响因子：
4.1
通讯作者：
Zhang, Yiying
中科院分区：
文献类型：
--
作者：
Zhang, Yiying
脂肪组织在能量平衡和代谢的调节中起着重要作用。两个特征将脂肪组织与其他器官区分开来-通过细胞大小和/或数量的增加而大大扩大其质量的能力，以及广泛的解剖分布。虽然脂肪组织功能受脂肪细胞大小和解剖位置的影响很大，但对脂肪细胞大小、数量和体脂分布的调节知之甚少。成熟脂肪组织或脂肪祖细胞的移植已被用于研究脂肪细胞的功能和生物合成。本文就移植的方法学考虑，包括供体材料的选择、移植部位和移植研究的时间，以及这些因素和血管、神经支配对移植物功能的影响作一综述。具体的研究也进行了审查，以说明在研究脂肪组织的功能和生物发生的脂肪组织移植的效用。重点是三个方面的研究：1）使用移植证明脂肪组织功能，如脂肪组织移植对受体动物的代谢和能量稳态的影响以及脂肪组织功能的储存库特异性差异; 2）使用移植解剖瘦素信号传导和性别对脂肪细胞大小的直接或细胞自主与间接或非细胞自主影响; 3）移植在脂肪祖细胞鉴定和谱系分析中的应用。最后，未来的应用移植在研究特定仓库脂肪细胞的生物发生，和遗传和激素的影响，性别和年龄对脂肪细胞的生物发生和功能进行了讨论。
Adipose tissue plays important roles in the regulation of energy homeostasis and metabolism. Two features distinguish adipose tissue from other organs - the ability to greatly expand its mass, via increases in cell size and/or number, and the wide anatomical distribution. While adipose tissue function is greatly affected by adipocyte size and anatomic location, regulations of adipocyte size, number, and body fat distribution are poorly understood. Transplantation of either mature adipose tissue or adipocyte progenitor cells has been used in studying adipocyte function and biogenesis. In this review, we will attempt to summarize methodological considerations for transplantation, including selections of donor material, transplantation site and the length of transplantation study, as well as effects of these factors and vascularization and innervation on the function of transplants. Specific studies are also reviewed to illustrate the utility of adipose tissue transplants in studying adipose tissue function and biogenesis. The focus is on studies in three areas: 1) use of transplants in demonstrating adipose tissue function, such as effects of adipose tissue transplants on metabolism and energy homeostasis of the recipient animals and depot-specific differences in adipose tissue function; 2) use of transplantation to dissect direct or cell-autonomous from indirect or non cell-autonomous effects of leptin signaling and sex on adipocyte size; 3) use of transplantation in the identification of adipocyte progenitor cells and lineage analysis. Finally, future applications of transplantation in studying depot-specific adipocyte biogenesis, and genetic and hormonal effects of sex and age on adipocyte biogenesis and function are discussed.
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影响因子：
4
作者：
Becú-Villalobos, D;Iglesias, AG;Libertun, C
通讯作者：
Libertun, C
影响因子：
3.6
作者：
BEYER, C;EUSTERSCHULTE, B;REISERT, I
通讯作者：
REISERT, I
影响因子：
7.7
作者：
Colombo, C;Cutson, JJ;Reitman, ML
通讯作者：
Reitman, ML
影响因子：
15.9
作者：
Crossno, Joseph T., Jr.;Majka, Susan M.;Klemm, Dwight J.
通讯作者：
Klemm, Dwight J.
DOI：
10.1098/rspb.1977.0014
发表时间：
1977-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
ASHWELL, M;MEADE, CJ;SOWTER, C
通讯作者：
SOWTER, C