Probiotic normalization of systemic inflammation in siblings of type 1 diabetes patients: an open-label pilot study.
Probiotic normalization of systemic inflammation in siblings of type 1 diabetes patients: an open-label pilot study.
复制标题
DOI：
10.1038/s41598-022-07203-6
复制
发表时间：
2022-02-28
影响因子：
4.6
通讯作者：
Hessner MJ
中科院分区：
文献类型：
--
作者：
Cabrera SM;Coren AT;Pant T;Ciecko AE;Jia S;Roethle MF;Simpson PM;Atkinson SN;Salzman NH;Chen YG;Hessner MJ
1型糖尿病（T1 D）的发病率增加，与生活方式的改变相吻合，这可能改变了肠道微生物群。与微生物抗原暴露一致的生态失调、肠道屏障功能障碍和全身炎症升高与T1 D易感性和进展相关。进行了一项为期6周的单臂开放性试验，以研究每日多菌株益生菌补充剂是否可以减少25名T1 D患者未受影响的兄弟姐妹的家族性炎症。益生菌补充剂的耐受性良好，这反映在参与者的高依从性和无不良事件。社区α和β多样性之间的前和后补充粪便采样没有改变。然而，LEfSe分析确定了补充后富集的家庭毛螺菌，生产商的抗炎短链脂肪酸丁酸。通过血浆诱导的转录测量全身炎症，并用基于基因本体的复合炎症指数（I.I.com）定量。补充后I.I.com显著降低，途径分析预测了多种炎症介质的抑制和IL 10 RA的激活。I.I.com中补充后减少最大的受试者在补充后表现出显著较低的CD 4 + CD 45 RO+（记忆）：CD 4 + CD 45 RA+（幼稚）T细胞比率。补充后IL-12 p40、IL-13、IL-15、IL-18、CCL 2和CCL 24血浆水平显著降低，而补充后丁酸盐水平倾向于高1.4倍。益生菌补充可能会改变T1 D的易感性和进展，并需要进一步研究。
The incidence of type 1 diabetes (T1D) has increased, coinciding with lifestyle changes that have likely altered the gut microbiota. Dysbiosis, gut barrier dysfunction, and elevated systemic inflammation consistent with microbial antigen exposure, have been associated with T1D susceptibility and progression. A 6-week, single-arm, open-label pilot trial was conducted to investigate whether daily multi-strain probiotic supplementation could reduce this familial inflammation in 25 unaffected siblings of T1D patients. Probiotic supplementation was well-tolerated as reflected by high participant adherence and no adverse events. Community alpha and beta diversity were not altered between the pre- and post-supplement stool samplings. However, LEfSe analyses identified post-supplement enrichment of the family Lachnospiraceae, producers of the anti-inflammatory short chain fatty acid butyrate. Systemic inflammation was measured by plasma-induced transcription and quantified with a gene ontology-based composite inflammatory index (I.I.com). Post-supplement I.I.com was significantly reduced and pathway analysis predicted inhibition of numerous inflammatory mediators and activation of IL10RA. Subjects with the greatest post-supplement reduction in I.I.com exhibited significantly lower CD4+ CD45RO+ (memory):CD4+ CD45RA+ (naïve) T-cell ratios after supplementation. Post-supplement IL-12p40, IL-13, IL-15, IL-18, CCL2, and CCL24 plasma levels were significantly reduced, while post-supplement butyrate levels trended 1.4-fold higher. Probiotic supplementation may modify T1D susceptibility and progression and warrants further study.
登录
查看更多内容
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
5
作者：
Chen, Y-G;Mordes, J. P.;Blankenhorn, E. P.;Kashmiri, H.;Kaldunski, M. L.;Jia, S.;Geoffrey, R.;Wang, X.;Hessner, M. J.
通讯作者：
Hessner, M. J.
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
7.7
作者：
Bonifacio E;Warncke K;Winkler C;Wallner M;Ziegler AG
通讯作者：
Ziegler AG
影响因子：
8.2
作者：
Cabrera SM;Chen YG;Hagopian WA;Hessner MJ
通讯作者：
Hessner MJ