Dynamic in vivo biocompatibility of angiogenic peptide amphiphile nanofibers.
Dynamic in vivo biocompatibility of angiogenic peptide amphiphile nanofibers.
复制标题
DOI：
10.1016/j.biomaterials.2009.07.063
复制
发表时间：
2009-10
期刊：
影响因子：
14
通讯作者：
Kirkpatrick, C. James
中科院分区：
文献类型：
--
作者：
Ghanaati, Shahram;Webber, Matthew J.;Unger, Ronald E.;Orth, Carina;Hulvat, James F.;Kiehna, Sarah E.;Barbeck, Mike;Rasic, Angela;Stupp, Samuel I.;Kirkpatrick, C. James
促进血管生成的生物材料在再生医学中具有巨大的潜力，可快速受损组织血运重建，移植细胞的存活以及慢性伤口的愈合。在这里评估了由肝素结合肽两亲物和硫酸乙酰肝素样糖胺聚糖形成的超分子纳米纤维，并使用背面皮肤折叠式腔室模型在这里评估，以动态监测该模型的纳米纤维凝胶和微纤维胶片之间的相互作用。我们将该模型与常规的皮下植入模型配对，以对凝胶和宿主组织之间的相互作用进行静态组织学评估。在静态分析中，发现含乙二醇的纳米纤维凝胶在组织中持续30天，并揭示出极好的生物相容性。令人惊讶的是，当纳米纤维凝胶生物降解时，我们观察到了从头血管化结缔组织的形成。在使用背面皮肤折室的动态实验中，该材料再次显示出良好的生物相容性，微循环的扩张最小，并且仅通过插入的荧光显微镜进行监测。背面皮肤模型的新应用证实了我们从传统的静态组织学中的发现，证明了该技术在动态评估材料的生物相容性时的潜在用途。使用这两种技术观察到的新血管化组织的生物相容性和开发都证明了这些血管生成材料对于多种再生策略的潜力。
Biomaterials that promote angiogenesis have great potential in regenerative medicine for rapid revascularization of damaged tissue, survival of transplanted cells, and healing of chronic wounds. Supramolecular nanofibers formed by self-assembly of a heparin-binding peptide amphiphile and heparan sulfate-like glycosaminoglycans were evaluated here using a dorsal skinfold chamber model to dynamically monitor the interaction between the nanofiber gel and the microcirculation, representing a novel application of this model. We paired this model with a conventional subcutaneous implantation model for static histological assessment of the interactions between the gel and host tissue. In the static analysis, the heparan sulfate-containing nanofiber gels were found to persist in the tissue for up to 30 days and revealed excellent biocompatibility. Strikingly, as the nanofiber gel biodegraded, we observed the formation of a de novo vascularized connective tissue. In the dynamic experiments using the dorsal skinfold chamber, the material again demonstrated good biocompatibility, with minimal dilation of the microcirculation and only a few adherent leukocytes, monitored through intravital fluorescence microscopy. The new application of the dorsal skinfold model corroborated our findings from the traditional static histology, demonstrating the potential use of this technique to dynamically evaluate the biocompatibility of materials. The observed biocompatibility and development of new vascularized tissue using both techniques demonstrates the potential of these angiogenesis-promoting materials for a host of regenerative strategies.
登录
查看更多内容
影响因子：
4.8
作者：
Herr, AB;Ornitz, DM;Waksman, G
通讯作者：
Waksman, G
影响因子：
4.7
作者：
Bull, SR;Guler, MO;Meade, TJ
通讯作者：
Meade, TJ
生物活性肽两亲物中凝胶化动力学的修饰。
影响因子：
14
作者：
Niece, Krista L.;Czeisler, Catherine;Sahni, Vibhu;Tysseling-Mattiace, Vicki;Pashuck, Eugene T.;Kessler, John A.;Stupp, Samuel I.
通讯作者：
Stupp, Samuel I.
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP
DOI：
10.1084/jem.138.4.745
发表时间：
1973-10-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Folkman J;Hochberg M
通讯作者：
Hochberg M