Mutation from arginine to lysine at the position 189 of hemagglutinin contributes to the antigenic drift in H3N2 swine influenza viruses.
Mutation from arginine to lysine at the position 189 of hemagglutinin contributes to the antigenic drift in H3N2 swine influenza viruses.
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DOI：
10.1016/j.virol.2013.08.004
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发表时间：
2013-11
期刊：
影响因子：
3.7
通讯作者：
Wan, Xiu-Feng
中科院分区：
文献类型：
--
作者：
Ye, Jianqiang;Xu, Yifei;Harris, Jillian;Sun, Hailiang;Bowman, Andrew S.;Cunningham, Fred;Cardona, Carol;Yoon, Kyoungjin J.;Slemons, Richard D.;Wan, Xiu-Feng
关键词：
先前在H3 N2猪A型流感病毒（IAV）中鉴定出两种不同的抗原簇，并将其命名为H3 N2 SIV-α和H3 N2 SIV-β（Journal of Virology 87（13），7655-7667）。在H3 N2 SIV-α和H3 N2 SIV-β fair分离株之间观察到血凝素（R189 K）的位置189处的一致突变。为了评估R189 K突变对H3 N2 SIV-α至H3 N2 SIV-β的抗原漂移的贡献，产生了具有189 R或189 K的四种重组病毒。抗原图谱显示，H3 N2 IAV血凝素中的R189 K突变有助于抗原漂移，将这些病毒分离为H3 N2 SIV-α至H3 N2 SIV-β。还发现这种R189 K突变有助于与几种雪貂血清的交叉反应，这些雪貂血清针对具有携带189 K的血凝素的历史人类IAV。这项研究表明，R189 K突变在猪和人H3 N2 IAV的抗原性中起着至关重要的作用，并且鉴定该抗原决定簇将有助于我们在流感监测中快速鉴定抗原变体。
Two distinct antigenic clusters were previously identified among the H3N2 swine influenza A viruses (IAVs) and were designated H3N2SIV-alpha and H3N2SIV-beta (, Journal of Virology 87(13), 7655-7667). A consistent mutation was observed at the position 189 of hemagglutinin (R189K) between H3N2SIV-alpha and H3N2SIV-beta fair isolates. To evaluate the contribution of R189K mutation to the antigenic drift from H3N2SIV-alpha to H3N2SIV-beta, four reassortant viruses with 189R or 189K were generated. The antigenic cartography demonstrated that the R189K mutation in the hemagglutinin of H3N2 IAV contributed to the antigenic drift, separating these viruses into H3N2SIV-alpha to H3N2SIV-beta. This R189K mutation was also found to contribute to the cross-reaction with several ferret sera raised against historical human IAVs with hemagglutinin carrying 189K. This study suggests that the R189K mutation plays a vital role in the antigenicity of swine and human H3N2 IAVs and identification of this antigenic determinant will help us rapidly identify antigenic variants in influenza surveillance.
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影响因子：
5.6
作者：
Cai, Zhipeng;Ducatez, Mariette F.;Yang, Jialiang;Zhang, Tong;Long, Li-Ping;Boon, Adrianus C.;Webby, Richard J.;Wan, Xiu-Feng
通讯作者：
Wan, Xiu-Feng
DOI：
10.1016/j.meegid.2010.08.010
发表时间：
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期刊：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.2
作者：
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通讯作者：
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