Deciphering the behaviour manipulation imposed by a virus on its parasitoid host: insights from a dual transcriptomic approach
Deciphering the behaviour manipulation imposed by a virus on its parasitoid host: insights from a dual transcriptomic approach
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破译病毒对其寄生宿主施加的行为操纵：来自双转录组学方法的见解
作者：
J. Varaldi;D. Lepetit
寄生虫强加的抽象行为操纵是一个令人着迷的现象，但我们的理解仍然非常有限。我们研究了一种病毒与攻击果蝇幼虫的寄生黄蜂Leptopilina Boulardi之间的相互作用。黄蜂通常避免在已经寄生的宿主中产卵(避免超寄生性)。相反，感染布拉细毛虫丝状病毒(LbFV)的雌性更容易重寄生。有趣的是，这种行为改变引起的宿主共享导致了病毒的水平传播，从而增加了它的适合度，但损害了黄蜂的适合度。为了更好地了解这种行为操纵的机制，我们通过RNA测序研究了LbFV和寄生蜂在腹部和头部的元转录组。我们发现，病毒转录本的丰度与黄蜂品系无关，但在不同组织之间存在很大差异。根据组织表达模式，我们确定了一组可能参与操纵过程的20个病毒基因。此外，我们确定了一组在病毒存在的情况下在腹部或头部失控的黄蜂基因，包括带有注释的与行为有关的基因(即钾通道蛋白)。这一数据集对寄生蜂的行为操纵和超寄生的遗传基础提供了新的见解。
Abstract Behaviour manipulation imposed by parasites is a fascinating phenomenon but our understanding is still very limited. We studied the interaction between a virus and the parasitic wasp Leptopilina boulardi that attacks Drosophila larvae. Wasps usually refrain to lay eggs into already parasitized hosts (superparasitism avoidance). On the contrary, females infected by the Leptopilina boulardi Filamentous Virus (LbFV) are much more incline to superparasitize. Interestingly, the host-sharing induced by this behaviour modification leads to the horizontal transmission of the virus, thus increasing its fitness at the expense of that of the wasp. To better understand the mechanisms underlying this behaviour manipulation, we studied by RNA sequencing the meta-transcriptome of LbFV and the parasitic wasp both in the abdomen and in the head. We found that the abundance of viral transcripts was independent of the wasp strain but strongly differed between tissues. Based on the tissue pattern of expression, we identified a set of 20 viral genes putatively involved in the manipulation process. In addition, we identified a set of wasp genes deregulated in the presence of the virus either in the abdomen or in the head, including genes with annotations suggesting involvement in behaviour (i.e. Potassium-channel protein). This dataset gives new insights into the behaviour manipulation and on the genetic basis of superparasitism in parasitoids.
蜜蜂 Kenyon 细胞受强直 GABA 受体电导调节。
影响因子：
2.5
作者：
Palmer MJ
通讯作者：
Palmer MJ