Assays of dioxins and dioxin-like compounds in actually contaminated soils using transgenic tobacco plants carrying a recombinant mouse aryl hydrocarbon receptor-mediated β-glucuronidase reporter gene expression system.
Assays of dioxins and dioxin-like compounds in actually contaminated soils using transgenic tobacco plants carrying a recombinant mouse aryl hydrocarbon receptor-mediated β-glucuronidase reporter gene expression system.
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DOI：
10.1080/03601234.2012.636575
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Ohkawa H
中科院分区：
文献类型：
--
作者：
Inui H;Gion K;Utani Y;Wakai T;Kodama S;Eun H;Kim YS;Ohkawa H
利用转小鼠芳烃受体XD 4V介导的β-葡萄糖醛酸酶（GUS）报告基因烟草XD 4V-26，对实际污染土壤中二恶英类化合物（包括多氯二苯并二恶英、多氯二苯并呋喃和共面多氯联苯（Co-PCBs））进行了检测。转基因烟草XD 4V-26在含有PCB 126的MS培养基上表现出明显的GUS诱导活性[毒性当量因子（TEF）= 0.1]，并与PCB 126浓度呈显著的剂量依赖关系。与此相反，PCB 169和PCB 180的TEF和未指定的TEF值分别为0.03，在与PCB 126相同的条件下，没有显着诱导GUS活性。当烟草植株在实际被二恶英和二恶英样化合物污染的土壤上栽培长达5周时，叶片中的GUS活性呈剂量依赖性增加，所述二恶英和二恶英样化合物是从用于处理生活和工业废物的焚烧炉的周边收集的。在这项检测中，这些植物明确检测到360 pg-TEQ g−1的二恶英和二恶英样化合物。GUS活性与植物体内二恶英类物质的TEQ值呈正相关。该分析不需要对实际污染的土壤样品进行任何提取和纯化过程。
The transgenic tobacco plant XD4V-26 carrying the recombinant mouse aryl hydrocarbon receptor XD4V-mediated β-glucuronidase (GUS) reporter gene expression system was used for assay of dioxins and dioxin-like compounds consisting of polychlorodibenzo-p-dioxins, polychlorinated dibenzofurans, and coplanar polychlorinated biphenyls (Co-PCBs) in actually contaminated soils. The transgenic tobacco plant XD4V-26 showed a significant dose-dependent induced GUS activity when cultured on MS medium containing PCB126 [toxic equivalency factor (TEF) = 0.1]. In contrast, PCB169 and PCB180, which have 0.03 of TEF and unassigned TEF values, respectively, did not significantly induce GUS activity under the same conditions as with PCB126. When the tobacco plants were cultivated for up to 5 weeks on actually contaminated soils with dioxins and dioxin-like compounds collected from the periphery of an incinerator used for disposal of life and industrial wastes, GUS activity in the leaves was dose-dependently increased. The plants clearly detected 360 pg-TEQ g−1 of dioxins and dioxin-like compounds in this assay. There was a positive correlation between GUS activity and TEQ value of dioxins and dioxin-like compounds in the plants. This assay does not require any extraction and purification processes for the actually contaminated soil samples.
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影响因子：
4.5
作者：
Chen, GS;Bunce, NJ
通讯作者：
Bunce, NJ
DOI：
10.1007/s00244-007-9036-y
发表时间：
2008-04-01
影响因子：
4
作者：
Kim, Yun-Seok;Eun, Heesoo;Katase, Takao
通讯作者：
Katase, Takao
影响因子：
4.3
作者：
Kodama, Stisumu;Okada, Kumiko;Ohkawa, Hideo
通讯作者：
Ohkawa, Hideo
DOI：
10.1080/03601234.2010.515166
发表时间：
2010-01-01
影响因子：
2
作者：
Shimazu, Sayuri;Ohta, Masaya;Ohkawa, Hideo
通讯作者：
Ohkawa, Hideo
影响因子：
2.4
作者：
White, Jason C.;Parrish, Zakia D.;Mattina, MaryJane Incorvia
通讯作者：
Mattina, MaryJane Incorvia