High speed random separation of microobject in microchip by laser manipulator and dielectrophoresis
High speed random separation of microobject in microchip by laser manipulator and dielectrophoresis
复制标题
激光机械臂和介电泳对微芯片中微小物体的高速随机分离
DOI：
--
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
A. Maeda
中科院分区：
文献类型：
--
作者：
F. Arai;M. Ogawa;T. Fukuda;K. Horio;T. Sone;K. Itoigawa;A. Maeda
我们开发了一种新的系统，用于在显微镜下通过光辐射压力和介电泳力控制的微流体装置中高速随机分离微物体，例如微生物。在微通道中可以快速分离任意单个微生物，即使溶液中存在大量的这些微生物。一旦目标微生物被捕获在激光操作器的焦点处，我们可以通过控制电场来容易地实现排除多余的微生物，同时保持目标被捕获在焦点处。为了实现高效的分离系统，我们采用微加工技术制作了一种新型的分离池。我们展示了我们的系统上的实验结果。我们还提出了间接操纵方法的微型工具，以避免直接的激光辐射的目标。一些初步的实验进行了验证。我们的方法是非常有用的目标微生物的纯培养，将是一个有前途的方法，为生物学家在发现一个新的微生物或生物修复。
We developed a new system for high speed random separation of a microobject, such as a microbe, in a microfluidic device by optical radiation pressure and dielectrophoretic force control under a microscope. An arbitrary single microbe can be isolated speedily in a microchannel, even though there are a large number of these microbes in solution. Once the target microbe is trapped at the focal point of the laser manipulator, we can easily realize exclusion of excess microbes by controlling the electric field, while keeping the target trapped at the focal point. To realize an efficient separation system, we made a new separation cell by microfabrication. We show experimental results on our system. We also propose indirect manipulation methods by the micro-tools to avoid direct laser radiation to the target. Some preliminary experiments are conducted to show the effectiveness. Our method is extremely useful in the pure cultivation of the targeted microbe and will be a promising method for biologists in discovering a new microbe or for bio-remediation.
影响因子：
3.4
作者：
Liang, H;Vu, KT;Berns, MW
通讯作者：
Berns, MW