Expulsion of Trichuris muris is associated with increased expression of angiogenin 4 in the gut and increased acidity of mucins within the goblet cell.
Expulsion of Trichuris muris is associated with increased expression of angiogenin 4 in the gut and increased acidity of mucins within the goblet cell.
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DOI：
10.1186/1471-2164-10-492
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发表时间：
2009-10-24
期刊：
影响因子：
4.4
通讯作者：
Else KJ
中科院分区：
文献类型：
--
作者：
D'Elia R;DeSchoolmeester ML;Zeef LA;Wright SH;Pemberton AD;Else KJ
小鼠鞭虫是人类感染胃肠道线虫鞭虫的宝贵模型。三个T已经研究了小鼠分离株，爱丁堡（E）、日本（J）和Sobreda（S）分离株。S分离物在C57 BL/6宿主内存活至慢性，而E和J在达到繁殖力之前被驱逐。尚不清楚S分离株如何在宿主中如此成功地存活。微阵列分析被用作鉴定基因的工具，所述基因的表达可以决定E和S T之间的排出动力学的差异。小鼠分离物。基因表达谱的明显差异早在感染后（p.i.）第7天就很明显。与来自S分离株感染的组织相比，来自E分离株感染的C57 BL/6小鼠的肠道组织中与免疫和防御反应相关的43个探针组上调，包括抗微生物蛋白血管生成素4（Ang 4）的信息。这导致了两种分离株感染后杯状细胞表型的明显差异。在S和E-感染的小鼠之间在感染早期鉴定的基因表达水平的差异进一步加深了我们对S分离株如何在C57 BL/6小鼠中比E分离株持续更长时间的认识。已经确定了为帮助驱逐而进行操纵的潜在新目标。此外，我们提供了一个潜在的新标记物的证据，涉及杯状细胞内的粘蛋白的酸度，可以预测感染的结果在几天内的寄生虫暴露。
Trichuris muris in the mouse is an invaluable model for infection of man with the gastrointestinal nematode Trichuris trichiura. Three T. muris isolates have been studied, the Edinburgh (E), the Japan (J) and the Sobreda (S) isolates. The S isolate survives to chronicity within the C57BL/6 host whereas E and J are expelled prior to reaching fecundity. How the S isolate survives so successfully in its host is unclear. Microarray analysis was used as a tool to identify genes whose expression could determine the differences in expulsion kinetics between the E and S T. muris isolates. Clear differences in gene expression profiles were evident as early as day 7 post-infection (p.i.). 43 probe sets associated with immune and defence responses were up-regulated in gut tissue from an E isolate-infected C57BL/6 mouse compared to tissue from an S isolate infection, including the message for the anti-microbial protein, angiogenin 4 (Ang4). This led to the identification of distinct differences in the goblet cell phenotype post-infection with the two isolates. Differences in gene expression levels identified between the S and E-infected mice early during infection have furthered our knowledge of how the S isolate persists for longer than the E isolate in the C57BL/6 mouse. Potential new targets for manipulation in order to aid expulsion have been identified. Further we provide evidence for a potential new marker involving the acidity of the mucins within the goblet cell which may predict outcome of infection within days of parasite exposure.
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影响因子：
2.2
作者：
D'Elia R;Else KJ
通讯作者：
Else KJ
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
1.6
作者：
Holloway, DE;Hares, MC;Acharya, KR
通讯作者：
Acharya, KR
影响因子：
30.5
作者：
Fallarino, F;Grohmann, U;Puccetti, P
通讯作者：
Puccetti, P
影响因子：
2.4
作者：
ELSE, K;WAKELIN, D
通讯作者：
WAKELIN, D