Biochemical and ultrastructural changes of skeletal muscle mitochondria after chronic electrical stimulation in rabbits
Biochemical and ultrastructural changes of skeletal muscle mitochondria after chronic electrical stimulation in rabbits
复制标题
慢性电刺激后兔骨骼肌线粒体的生化和超微结构变化
DOI：
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
D. Pette
中科院分区：
文献类型：
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作者：
H. Reichmann;Hans Hoppeler;O. Mathieu‐costello;F. Bergen;D. Pette
本研究的目的是跟踪和相关的结构和生化指标的能量代谢的变化，在慢性电刺激兔胫骨前肌muscle.In肌肉的浅表部分，5至6倍的柠檬酸循环和脂肪酸氧化的酶活性的刺激后28天发生的增加。酶活性的变化在深层，更氧化的肌肉部分相对较小。因此，柠檬酸循环酶的水平在最长的刺激期后在肌肉的浅表和深部变得相似。除了与柠檬酸循环酶平行增加的己糖激酶外，糖酵解酶减少2至3倍。肌肉质量和纤维大小保持不变，而毛细血管密度和毛细血管纤维比增加2倍。总线粒体的体积密度以类似于柠檬酸循环的酶的变化的方式增加（在肌肉的浅表部分中为7倍，在深部部分中为3.5倍），并且因此接近在心肌中发现的值。酮体利用和线粒体甘油磷酸氧化酶的酶活性的不成比例的变化表明线粒体群体内的质的变化。然而，肌膜下的原纤维间线粒体的比例，以及每单位体积的线粒体内膜面积保持不变。同样，细胞内的脂质沉积物保持不变，与stimulation.It的结论是，有一个很好的组织氧化能力的形态测量和生化测量之间的协议。
The purpose of the present investigation was to follow and correlate changes of structural and biochemical markers of energy metabolism during chronic electrical stimulation of tibialis anterior muscle in rabbits.In the superficial portion of the muscle, 5 to 6-fold increases occurred in enzyme activities of the citric acid cycle and of fatty acid oxidation after 28 days of stimulation. Enzyme activity changes in the deep, more oxidative part of the muscle were relatively smaller. Consequently, levels of the citric acid cycle enzymes became similar in superficial and deep parts of the muscle after the longest stimulation periods. With the exception of hexokinase, which increased in parallel with the citric acid cycle enzymes, glycolytic enzymes decreased 2 to 3-fold. Muscle mass and fibre size remained unchanged, while capillary density and capillary to fiber ratio increased 2-fold. The volume density of total mitochondria increased in a fashion similar to the changes of the enzymes of the citric acid cycle (7-fold in superficial and 3.5-fold in deep parts of the muscle) and, thus, approached values found in heart muscle. Disproportionate changes in enzyme activities of ketone body utilisation and of mitochondrial glycerolphosphate oxidase indicated qualitative changes within the mitochondrial population. However, the proportion of subsarcolemmal to interfibrillar mitochondria, as well as the area of inner mitochondrial membrane per unit volume of mitochondrion remained unchanged. Similarly, intracellular lipid deposits remained unchanged with stimulation.It is concluded that there is an excellent agreement between morphometric and biochemical measurements of tissue oxidative capacity.
影响因子：
3.9
作者：
DAVIES, KJA;PACKER, L;BROOKS, GA
通讯作者：
BROOKS, GA