Overexpression of mitochondrial γCAL1 reveals a unique photoprotection mechanism in intertidal resurrection red algae through decreasing photorespiration
Overexpression of mitochondrial γCAL1 reveals a unique photoprotection mechanism in intertidal resurrection red algae through decreasing photorespiration
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线粒体γCAL1的过度表达揭示了潮间带复活红藻通过减少光呼吸的独特光保护机制
DOI：
10.1016/j.algal.2022.102766
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发表时间：
2022-07
期刊：
影响因子：
--
通讯作者：
Wang Guangce
中科院分区：
文献类型：
--
作者：
Shao zhizhuo;Xie Xiujun;Liu Xueying;Zheng Zhenbing;Huan Li;Zhang Baoyu;Wang Guangce
目前还没有明确的证据表明，在含有藻胆体的物种中，包括红藻和蓝藻，存在一个有效的叶黄素循环。特别是，潮间带红藻受到不利条件，如强光。(HL)周期性的压力。因此，红藻如何避免光诱导的伤害从而达到光保护的目的引起了人们的极大兴趣。光合放氧（POE）和光系统II（PSII）活性分析结果表明，条斑新光甲（Neopyropia yezoensis）是一种重要的潮间带经济红藻，在超出其生长所需的光照强度下，仍具有较高的光合活性。然而，这样的HL强度迫使许多藻类经历光抑制。通过系统分析，我们发现线粒体中的γ型碳酸酐酶样1（γ CAL 1）蛋白在光保护中起关键作用。我们认为HL光呼吸产生的CO2通过γ CAL 1转化为碳酸氢盐，阻止CO2逃逸。碳酸二氢盐被转化回CO2，CO2在核酮糖-1，5-二磷酸羧化酶/加氧酶（Rubisco）附近富集，导致CO2分压增加。为了验证这一结果，我们构建了γ CAL 1过表达菌株。有趣的是，与野生型（WT）相比，转基因株系中光呼吸强度降低，这与在高等植物中报道的结果一致。基于上述结果，很明显，促进卡尔文循环的效率是一个重要的机制。yezoensis对HL胁迫的抗性。在这一过程中，光呼吸可提供充足的CO2，γ CAL 1直接参与其中。因此，本研究为揭示含藻胆体的复活藻耐受过量光能的策略提供了新的证据
There is no clear evidence of an operative xanthophyll cycle in species containing phycobilisomes, including red.algae and cyanobacteria. In particular, intertidal red algae are subjected to adverse conditions such as high light.(HL) stress periodically. Therefore, how red algae avert light-induced damage for photoprotection has attracted.much interest. Photosynthetic oxygen evolution (POE) and photosystem II (PSII) activity analysis showed that.Neopyropia yezoensis, an important economic red algae in the intertidal zone, possessed relatively high photosynthetic activity despite being under light intensity beyond the required of growth. However, such HL intensity.has forced many algae to experience photoinhibition. Through systematic analysis, we found that gamma-type.carbonic anhydrase-like1 (γCAL1) protein in mitochondria plays a key photoprotective role. We propose that.the CO2 produced by photorespiration under HL is transformed into bicarbonate, through γCAL1, to prevent the.escape of CO2. Bicarbonate is converted back into CO2, which is enriched near the Ribulose-1,5-bisphosphate.carboxylase/oxygenase (Rubisco), causing an increase in the partial pressure of CO2. To verify this result, we.constructed γCAL1 overexpressing strains. It is interesting to discover the decreasing photorespiration flux in the.transgenic strain, compared with the wild type (WT), is consistent with the fndings reported in higher plants..Based on the above results, it is clear that promoting the effciency of the Calvin cycle is an important mechanism.for N. yezoensis to resist HL stress. During this process, suffcient CO2 can be supplied by photorespiration, where.γCAL1 directly participates. Therefore, our research provides new evidence for revealing the strategies of.phycobilisome-containing resurrection algae tolerating excess light energy
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影响因子：
3
作者：
Hirata, Ryo;Takahashi, Megumu;Mikami, Koji
通讯作者：
Mikami, Koji
影响因子：
3.1
作者：
EILERS, PHC;PEETERS, JCH
通讯作者：
PEETERS, JCH
影响因子：
--
作者：
W. Zhou;Linwen He;Fang Yang;A. Lin;Baoyu Zhang;J. Niu;Guangce Wang
通讯作者：
W. Zhou;Linwen He;Fang Yang;A. Lin;Baoyu Zhang;J. Niu;Guangce Wang
影响因子：
3.7
作者：
U. Nitschke;S. Connan;D. B. Stengel
通讯作者：
U. Nitschke;S. Connan;D. B. Stengel
影响因子：
3
作者：
Uji, Toshiki;Takahashi, Megumu;Mikami, Koji
通讯作者：
Mikami, Koji