The giant diploid faba genome unlocks variation in a global protein crop.
The giant diploid faba genome unlocks variation in a global protein crop.
复制标题
巨型二倍体Faba基因组解锁了全球蛋白质作物的变化。
DOI：
10.1038/s41586-023-05791-5
复制
发表时间：
2023-03
期刊：
影响因子：
64.8
通讯作者：
Andersen, Stig Uggerhoj
中科院分区：
文献类型：
--
作者：
Jayakodi, Murukarthick;Golicz, Agnieszka A.;Kreplak, Jonathan;Fechete, Lavinia, I;Angra, Deepti;Bednar, Petr;Bornhofen, Elesandro;Zhang, Hailin;Boussageon, Raphael;Kaur, Sukhjiwan;Cheung, Kwok;Cizkova, Jana;Gundlach, Heidrun;Hallab, Asis;Imbert, Baptiste;Keeble-Gagnere, Gabriel;Koblizkova, Andrea;Kobrlova, Lucie;Krejci, Petra;Mouritzen, Troels W.;Neumann, Pavel;Nadzieja, Marcin;Nielsen, Linda Kaergaard;Novak, Petr;Orabi, Jihad;Padmarasu, Sudharsan;Robertson-Shersby-Harvie, Tom;Robledillo, Laura Avila;Schiemann, Andrea;Tanskanen, Jaakko;Toronen, Petri;Warsame, Ahmed O.;Wittenberg, Alexander H. J.;Himmelbach, Axel;Aubert, Gregoire;Courty, Pierre-Emmanuel;Dolezel, Jaroslav;Holm, Liisa U.;Janss, Luc L.;Khazaei, Hamid;Macas, Jiri;Mascher, Martin;Smykal, Petr;Snowdon, Rod J.;Stein, Nils;Stoddard, Frederick L.;Stougaard, Jens;Tayeh, Nadim;Torres, Ana M.;Usadel, Bjorn;Schubert, Ingo;O'Sullivan, Donal Martin;Schulman, Alan H.;Andersen, Stig Uggerhoj
在目前富含肉类的饮食中，增加当地生产的植物蛋白的比例可以大大减少温室气体排放和生物多样性的丧失。然而，由于在农艺价值上缺乏与大豆相当的冷季豆科植物，植物蛋白的生产受到了阻碍。蚕豆（Vicia Faba L.）具有高产潜力，适合在温带地区种植，但基因组资源匮乏。在这里，我们报道了蚕豆基因组的高质量染色体尺度组装，并表明它已经扩展到巨大的13 Gb大小，通过扩增率和消除反转录转座子和卫星重复之间的不平衡。基因和重组事件均匀地分散在染色体上，考虑到基因组的大小，基因空间非常紧凑，尽管有大量的拷贝数变异是由串联复制驱动的。为了演示基因组序列的实际应用，我们开发了一种靶向基因分型分析方法，并使用高分辨率全基因组关联分析来解剖种子大小和门部颜色的遗传基础。提供的资源构成了一个基于基因组学的蚕豆育种平台，使育种家和遗传学家能够加速改善地中海、亚热带和北温带农业生态区的可持续蛋白质生产。利用蚕豆基因组的高质量染色体尺度组装，探索了种子大小和门部颜色的遗传基础。
Increasing the proportion of locally produced plant protein in currently meat-rich diets could substantially reduce greenhouse gas emissions and loss of biodiversity. However, plant protein production is hampered by the lack of a cool-season legume equivalent to soybean in agronomic value. Faba bean (Vicia faba L.) has a high yield potential and is well suited for cultivation in temperate regions, but genomic resources are scarce. Here, we report a high-quality chromosome-scale assembly of the faba bean genome and show that it has expanded to a massive 13 Gb in size through an imbalance between the rates of amplification and elimination of retrotransposons and satellite repeats. Genes and recombination events are evenly dispersed across chromosomes and the gene space is remarkably compact considering the genome size, although with substantial copy number variation driven by tandem duplication. Demonstrating practical application of the genome sequence, we develop a targeted genotyping assay and use high-resolution genome-wide association analysis to dissect the genetic basis of seed size and hilum colour. The resources presented constitute a genomics-based breeding platform for faba bean, enabling breeders and geneticists to accelerate the improvement of sustainable protein production across the Mediterranean, subtropical and northern temperate agroecological zones. Using a high-quality chromosome-scale assembly of the faba bean genome, the genetic basis of seed size and hilum colour is explored.
登录
查看更多内容
影响因子：
3.7
作者：
Chang W;Jääskeläinen M;Li SP;Schulman AH
通讯作者：
Schulman AH
影响因子：
18
作者：
Björnsdotter E;Nadzieja M;Chang W;Escobar-Herrera L;Mancinotti D;Angra D;Xia X;Tacke R;Khazaei H;Crocoll C;Vandenberg A;Link W;Stoddard FL;O'Sullivan DM;Stougaard J;Schulman AH;Andersen SU;Geu-Flores F
通讯作者：
Geu-Flores F
影响因子：
48
作者：
Cheng H;Concepcion GT;Feng X;Zhang H;Li H
通讯作者：
Li H
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
7.4
作者：
De La Torre, Amanda R.;Birol, Inanc;Bohlmann, Joerg
通讯作者：
Bohlmann, Joerg