A relationship between proteinase activity and clinical parameters in the treatment of periodontal disease.
A relationship between proteinase activity and clinical parameters in the treatment of periodontal disease.
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牙周病治疗中蛋白酶活性与临床参数之间的关系。
DOI：
10.1111/j.1600-051x.1998.tb02491.x
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发表时间：
1998
影响因子：
6.7
通讯作者：
Russell,CM
中科院分区：
文献类型：
--
作者：
Mailhot,JM;Potempa,J;Stein,SH;Travis,J;Sterrett,JD;Hanes,PJ;Russell,CM
本研究的目的是确定生物化学测定的有效性，该测定测量龈沟液（GCF）中的蛋白水解酶活性，并将该酶活性与传统上用于牙周炎检测的临床参数相关联。对8名牙周炎受试者进行了一项临床试验，其中≥4个部位表现出≥5 mm的附着丧失和≥5 mm的探诊深度，并在探诊时出血。对每名受试者进行牙菌斑指数，然后在表现出附着和探测深度丧失的部位进行GCF采样。分析GCF在存在（BAPNA w/gly-gly）和不存在（BAPNA w/o gly-gly）甘氨酰-甘氨酸的情况下对苯甲酰-L-精氨酸-对硝基苯胺的活性，以及对MeOSuc-Ala-Ala-Pro-瓦尔-pNA和Suc-Ala-Ala-Pro-Phe-pNA的中性粒细胞丝氨酸蛋白酶活性（分别为弹性蛋白酶和组织蛋白酶G）。随后，进行牙龈指数，使用恒力探针记录附着水平和探诊深度，并记录探诊出血。采用裂口设计，将一半口腔随机分配到以下治疗组：A组，一半口腔接受洁治/根面平整和抛光; B组，一半口腔不接受治疗（对照）。受试者接受治疗，然后指导刷牙和齿间清洁。4周后，受试者返回接受菌斑指数; GCF采样、牙龈指数、附着水平、探诊深度和探诊出血，如上所述。使用patred Student检验，结果表明治疗部位的BAPNA w/gly-gly显著低于未治疗对照部位（p=0.05）。没有发现这种相关性的其他活动，包括中性粒细胞丝氨酸蛋白酶，这被证明是发生在GCF中的自由，蛋白水解活性形式。此外，探测深度（p= 0.03）减少了1.3 mm，附着水平（p=0.02）增加了0.7 mm，检测到显著的治疗效果。通过BAPNA w/gly-gly的显著降低检测到的治疗牙周炎部位的牙龈炎可能被证明是牙周病活动的有价值的标志物。
The objective of this research was to determine the effectiveness of a biochemical assay which measures proteolytic enzyme activity in gingival crevicular fluid (GCF) and to relate this enzyme activity to clinical parameters traditionally utilized for periodontitis detection. A clinical trial was conducted on 8 periodontitis subjects with ≥4 sites exhibiting a loss of attachment of ≥5 mm and probing depths of ≥5 mm with bleeding on probing. On each subject, a plaque index was performed, followed by GCF sampling at those sites which exhibited a loss of attachment and probing depths. GCF was analyzed for activity against benzoyl‐L‐arginine‐p‐nitroanilide in the presence (BAPNA w/gly‐gly) and the absence (BAPNA w/o gly‐gly) of glycyl‐glycine and against MeOSuc‐Ala‐Ala‐Pro‐Val‐pNA and Suc‐Ala‐Ala‐Pro‐Phe‐pNA for neutrophil serine proteinases activity (elastase and cathepstn G, respectively). Subsequently, a gingival index was performed, attachment levels and probing depths were recorded using a constant force probe with bleeding on probing being noted. A split‐mouth design was employed and half mouths were randomly assigned to the following treatment groups: group A, half of the mouth received scaling/root planing and polishing: group B, half of the mouth received no treatment (control). Subjects were treated, then instructed on toothbrushing and interdental cleaning. After 4 weeks, subjects returned to receive a plaque index; GCF sampling, gingival index, attachment levels, probing depths and bleeding on probing as described above. Using a patred Studentt‐test, the findings suggest that BAPNA w/gly‐gly was significantly less in treatment sites than in non‐treated control sites (p=0.05). No such correlation was found for other activities, including neutrophil serine proteinases which were shown to occur in GCF in free, proteolytically active forms. In addition, significant treatment effects were detected for probing depths (p= 0.03) which reduced by 1.3 mm and attachment levels (p=0.02) which gained 0.7 mm. The reduction ofP. gingivalisfrom treated periodontitis sites as detected by a significant decrease in BAPNA w/gly‐gly may prove to be a valuable marker for periodontal disease activity.
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DOI：
10.1016/s0021-9258(19)37046-2
发表时间：
1992-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Wingrove;R. Discipio;Z. Chen;J. Potempa;James Travis;T. E. Hugli
通讯作者：
J. Wingrove;R. Discipio;Z. Chen;J. Potempa;James Travis;T. E. Hugli
影响因子：
6.7
作者：
MCCULLOCH, CAG
通讯作者：
MCCULLOCH, CAG
影响因子：
6.7
作者：
GOODSON, JM;TANNER, ACR;SOCRANSKY, SS
通讯作者：
SOCRANSKY, SS
沟液中的酶活性用于检测和预测慢性成人牙周炎患者的临床附着丧失。
影响因子：
4.3
作者：
Lamster,IB;Oshrain,RL;Harper,DS;Celenti,RS;Hovliaras,CA;Gordon,JM
通讯作者：
Gordon,JM
影响因子：
2
作者：
LOE, HARALD;SILNESS, JOHN
通讯作者：
SILNESS, JOHN