Leishmania parasitophorous vacuoles interact continuously with the host cell's endoplasmic reticulum; parasitophorous vacuoles are hybrid compartments.
Leishmania parasitophorous vacuoles interact continuously with the host cell's endoplasmic reticulum; parasitophorous vacuoles are hybrid compartments.
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DOI：
10.1111/j.1462-5822.2010.01483.x
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发表时间：
2010-10
影响因子：
3.4
通讯作者：
Kima PE
中科院分区：
文献类型：
--
作者：
Ndjamen B;Kang BH;Hatsuzawa K;Kima PE
产生表达用GFP标记的代表性内质网（ER）分子的巨噬细胞以评估ER分子向利什曼原虫寄生空泡（PV）的募集。90%以上的PV携带L. pifanoi或L. donovani寄生虫将钙连接蛋白募集到它们的PV膜（PVM）。等比例的PV还募集膜相关可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体（SNARE）Sec 22 b。这两种ER分子似乎在新生PV形成的早期就被招募。感染的Sec 22 b/YFP表达细胞的电子显微镜分析证实Sec 22 b被招募到利什曼原虫PV。与PV相反，发现不超过20%的含有酵母多糖颗粒的吞噬体将钙连接蛋白或Sec 22 b募集到其限制性吞噬体膜。利用蓖麻毒素进入细胞胞质溶胶的逆行途径进一步了解ER-PV相互作用。蓖麻毒素被传递到与蓖麻毒素一起孵育的感染细胞中的PV。用布雷菲德菌素A孵育细胞阻断蓖麻毒素向PV的转移。这意味着运输到ER的分子被转移到PV。此外，结果表明，PV是由宿主ER和内吞途径组分组成的混合隔室。
Macrophages that express representative endoplasmic reticulum (ER) molecules tagged with GFP were generated to assess the recruitment of ER molecules to Leishmania parasitophorous vacuoles (PVs). More than 90% of PVs harboring L. pifanoi or L. donovani parasites recruited calnexin, to their PV membrane (PVM). An equivalent proportion of PVs also recruited the membrane associated soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE), Sec22b. Both ER molecules appeared to be recruited very early in the formation of nascent PVs. Electron microscopy analysis of infected Sec22b/YFP expressing cells confirmed that Sec22b was recruited to Leishmania PVs. In contrast to PVs, it was found that no more than 20% of phagosomes that harbored Zymosan particles recruited calnexin or Sec22b to their limiting phagosomal membrane. The retrograde pathway that ricin employs to access the cell cytosol was exploited to gain further insight into ER-PV interactions. Ricin was delivered to PVs in infected cells incubated with ricin. Incubation of cells with Brefeldin A blocked the transfer of ricin to PVs. This implied that molecules that traffic to the ER are transferred to PVs. Moreover the results show that PVs are hybrid compartments that are composed of both host ER and endocytic pathway components.
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影响因子：
64.8
作者：
Houde, M;Bertholet, S;Desjardins, M
通讯作者：
Desjardins, M
DOI：
10.1083/jcb.124.5.677
发表时间：
1994-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Desjardins M;Huber LA;Parton RG;Griffiths G
通讯作者：
Griffiths G
影响因子：
2.9
作者：
Kang, Byung-Ho;Staehelin, L. Andrew
通讯作者：
Staehelin, L. Andrew
影响因子：
3.3
作者：
Hatsuzawa, Kiyotaka;Hashimoto, Hitoshi;Wada, Ikuo
通讯作者：
Wada, Ikuo
DOI：
10.1083/jcb.131.6.1715
发表时间：
1995-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Nakamura N;Rabouille C;Watson R;Nilsson T;Hui N;Slusarewicz P;Kreis TE;Warren G
通讯作者：
Warren G