Enhanced bacterial adhesion on surfaces pretreated with fibrinogen and fibronectin.

The effect of certain plasma proteins on the adhesion of Pseudomonas aeruginosa and Staphylococcus epidermidis on polyurethane, polyvinylchloride, or glass was investigated. Test surfaces were treated with serum, plasma, albumin, immunoglobulin G, fibrinogen, or fibronectin. Using a specially designed test chamber, surfaces previously treated with test proteins were incubated with bacterial suspension. During the experiment, the test chamber was placed on a rotator to prevent settling of bacteria. At the end of the experiment, each test well was rinsed repeatedly to remove non-adherent bacteria. The number of bacteria adherent to the test surfaces was quantitated by a combination of methods including microscopic counting of cells, scintillation counting and autoradiography. It was noted that a greater number of bacteria adhered to surfaces coated with fibrinogen or fibronectin whereas surfaces treated with serum showed reduced bacterial adhesion. The inhibitory effect of serum appeared more pronounced with S. epidermidis when compared with P. aeruginosa under identical experimental conditions. Scanning electron microscopy revealed that adherent bacteria were randomly distributed on the test surfaces and appeared to replicate while still adherent. These observations suggested that bacterial adhesion to biomaterials can be significantly influenced by the composition of the adsorbed proteins at the interface.

研究了某些血浆蛋白对铜绿假单胞菌和表皮葡萄球菌在聚氨酯、聚氯乙烯或玻璃上黏附的影响。用血清、血浆、白蛋白、免疫球蛋白G、纤维蛋白原或纤连蛋白处理测试表面。使用专门设计的测试室，将先前用测试蛋白处理过的表面与细菌悬液一起培养。在实验过程中，将测试室放在旋转器上以防止细菌沉降。实验结束时，反复冲洗每个测试孔以去除未黏附的细菌。通过多种方法相结合对黏附在测试表面的细菌数量进行定量，这些方法包括细胞显微镜计数、闪烁计数和放射自显影。注意到更多的细菌黏附在涂有纤维蛋白原或纤连蛋白的表面，而用血清处理过的表面细菌黏附减少。在相同的实验条件下，与铜绿假单胞菌相比，血清对表皮葡萄球菌的抑制作用更为明显。扫描电子显微镜显示，黏附的细菌随机分布在测试表面，并且似乎在黏附的同时进行复制。这些观察结果表明，细菌对生物材料的黏附可受到界面处吸附蛋白质组成的显著影响。