Downstream reactions and engineering in the microbially reconstituted pathway for Taxol.
Downstream reactions and engineering in the microbially reconstituted pathway for Taxol.
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DOI：
10.1007/s00253-012-4016-1
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发表时间：
2012-05
影响因子：
5
通讯作者：
Pfeifer, Blaine A.
中科院分区：
文献类型：
--
作者：
Jiang, Ming;Stephanopoulos, Gregory;Pfeifer, Blaine A.
紫杉醇（Bristol-Myers Squibb的商标产品）是一种复杂的类异戊二烯天然产物，其显示出有效的抗癌活性。紫杉醇最初从太平洋紫杉树（短叶红豆杉）中分离出来，已经通过依赖于植物来源的生物合成的过程大量生产。最近，已经有替代的努力，通过技术上方便的微生物宿主，提供无与伦比的生长动力学和工程潜力，以重建生物合成过程。由于需要成功引入负责最终生物合成的显著外源酶促步骤，使得这种方法具有挑战性。然而，这样做提供了设计更有效和更经济的生产工艺的潜力，以及设计和生产具有增强性能的定制模拟化合物的机会。这篇小型综述将特别关注异源生物合成，因为它适用于紫杉醇，重点是与引入和重建最终生物活性所需的下游反应步骤相关的挑战。
Taxol (a trademarked product of Bristol-Myers Squibb) is a complex isoprenoid natural product which has displayed potent anticancer activity. Originally isolated from the Pacific yew tree (Taxus brevifolia), Taxol has been mass-produced through processes reliant on plant-derived biosynthesis. Recently, there have been alternative efforts to reconstitute the biosynthetic process through technically convenient microbial hosts, which offer unmatched growth kinetics and engineering potential. Such an approach is made challenging by the need to successfully introduce the significantly foreign enzymatic steps responsible for eventual biosynthesis. Doing so, however, offers the potential to engineer more efficient and economical production processes and the opportunity to design and produce tailored analog compounds with enhanced properties. This mini review will specifically focus on heterologous biosynthesis as it applies to Taxol with an emphasis on the challenges associated with introducing and reconstituting the downstream reaction steps needed for final bioactivity.
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影响因子：
--
作者：
Chau, M;Jennewein, S;Croteau, R
通讯作者：
Croteau, R
DOI：
10.1126/science.1191652
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ajikumar PK;Xiao WH;Tyo KE;Wang Y;Simeon F;Leonard E;Mucha O;Phon TH;Pfeifer B;Stephanopoulos G
通讯作者：
Stephanopoulos G
影响因子：
3.8
作者：
Jennewein, S;Park, H;Croteau, RB
通讯作者：
Croteau, RB
DOI：
10.1073/pnas.89.22.10817
发表时间：
1992-11-15
影响因子：
11.1
作者：
FISHER, CW;SHET, MS;ESTABROOK, RW
通讯作者：
ESTABROOK, RW
影响因子：
3.9
作者：
Jennewein, S;Rithner, CD;Croteau, R
通讯作者：
Croteau, R