NOD dendritic cells stimulated with Lactobacilli preferentially produce IL-10 versus IL-12 and decrease diabetes incidence.
NOD dendritic cells stimulated with Lactobacilli preferentially produce IL-10 versus IL-12 and decrease diabetes incidence.
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DOI：
10.1155/2011/630187
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发表时间：
2011
影响因子：
--
通讯作者：
Alard P
中科院分区：
文献类型：
--
作者：
Manirarora JN;Parnell SA;Hu YH;Kosiewicz MM;Alard P
来自NOD小鼠的树突状细胞（DC）产生高水平的IL-12，其诱导参与糖尿病发展的产生IFNγ的T细胞。我们建议利用微生物诱导耐受性DC的能力来消除促炎过程并预防糖尿病的发展。用乳酸杆菌（靶向TLR 2的非致病性细菌）或来自金黄色葡萄球菌的脂磷壁酸（LTA）（TLR 2激动剂）刺激NOD DC。LTA处理的DC产生比IL-10多得多的IL-12，并且当转移到NOD小鼠中时加速糖尿病的发展。相反，用L.干酪素有利于IL-10的产生，而不是IL-12，它们的转移降低了疾病的发病率，抗IL-10 R抗体恢复了疾病的发病率。这些数据表明L.酪蛋白可以通过产生抗炎细胞因子IL-10诱导NOD DC产生更耐受性的表型。评估DC的IL-10和IL-12的相对产生可能是鉴定具有治疗潜力的药剂的非常有用的手段。
Dendritic cells (DCs) from NOD mice produced high levels of IL-12 that induce IFNγ-producing T cells involved in diabetes development. We propose to utilize the microorganism ability to induce tolerogenic DCs to abrogate the proinflammatory process and prevent diabetes development. NOD DCs were stimulated with Lactobacilli (nonpathogenic bacteria targeting TLR2) or lipoteichoic acid (LTA) from Staphylococcus aureus (TLR2 agonist). LTA-treated DCs produced much more IL-12 than IL-10 and accelerated diabetes development when transferred into NOD mice. In contrast, stimulation of NOD DCs with L. casei favored the production of IL-10 over IL-12, and their transfer decreased disease incidence which anti-IL-10R antibodies restored. These data indicated that L. casei can induce NOD DCs to develop a more tolerogenic phenotype via production of the anti-inflammatory cytokine, IL-10. Evaluation of the relative production of IL-10 and IL-12 by DCs may be a very useful means of identifying agents that have therapeutic potential.
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影响因子：
15.3
作者：
Geijtenbeek, TBH;van Vliet, SJ;Koppel, EA;Sanchez-Hernandez, M;Vandenbroucke-Grauls, CMJE;Appelmelk, B;van Kooyk, Y
通讯作者：
van Kooyk, Y
影响因子：
4.4
作者：
Christensen, HR;Frokiær, H;Pestka, JJ
通讯作者：
Pestka, JJ
影响因子：
15.9
作者：
Dillon, S;Agrawal, S;Pulendran, B
通讯作者：
Pulendran, B
DOI：
10.1111/j.1749-6632.1996.tb52670.x
发表时间：
1996-01-01
期刊：
INERLEUKIN 12: CELLULAR AND MOLECULAR IMMUNOLOGY OF AN IMPORTANT REGULATORY CYTOKINE
影响因子：
--
作者：
Adorini, L;Gregori, S;Trembleau, S
通讯作者：
Trembleau, S
影响因子：
6.4
作者：
Larsen, Jeppe Madura;Benn, Christine Stabell;Yazdanbakhsh, Maria
通讯作者：
Yazdanbakhsh, Maria