Right in two: capabilities of ion mobility spectrometry for untargeted metabolomics.
Right in two: capabilities of ion mobility spectrometry for untargeted metabolomics.
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DOI：
10.3389/fmolb.2023.1230282
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发表时间：
2023
影响因子：
5
通讯作者：
中科院分区：
文献类型：
--
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这篇简短的综述集中于当前和新兴的分离技术为质谱学代谢组学提供的机会。代谢组学中分离技术的目的主要是降低所研究的非均相系统的复杂性，并通过增加对低丰度代谢物定量的敏感性来提供浓缩浓缩。因此，各种各样的分离系统，从柱化学到溶剂组成和多维分离，已经在该领域中得到应用。多维分离是蛋白质组学应用和气相色谱-质谱学中的常用方法，允许正交分离以进一步降低分析复杂性并扩大峰容量。随着第一维分离和第二维分离，这些应用程序导致运行时间呈指数级增长。多维液相色谱增加了代谢组学中的峰容量，与运行更多样本或重复并增加统计置信度的潜力相比，意味着这些方法的吸收一直很少。相比之下，在过去的15年里，离子迁移率光谱在分辨率和灵敏度方面取得了重大进展，以至于基于碰撞截面的分析物的高分辨率分离接近于色谱分离，而灵敏度损失最小。此外，离子迁移率分离可以在色层时间刻度上进行，而几乎不会减少仪器的占空比。本文对离子迁移率分离与高效液相色谱分离进行了比较，重点介绍了离子迁移率分离在代谢组学中的应用历史，概述了该领域的最新进展，并对该技术的发展前景进行了展望。“只要有一个，你就一定会分掉。正中两分“，詹姆斯·梅纳德·基南。
This mini review focuses on the opportunities provided by current and emerging separation techniques for mass spectrometry metabolomics. The purpose of separation technologies in metabolomics is primarily to reduce complexity of the heterogeneous systems studied, and to provide concentration enrichment by increasing sensitivity towards the quantification of low abundance metabolites. For this reason, a wide variety of separation systems, from column chemistries to solvent compositions and multidimensional separations, have been applied in the field. Multidimensional separations are a common method in both proteomics applications and gas chromatography mass spectrometry, allowing orthogonal separations to further reduce analytical complexity and expand peak capacity. These applications contribute to exponential increases in run times concomitant with first dimension fractionation followed by second dimension separations. Multidimensional liquid chromatography to increase peak capacity in metabolomics, when compared to the potential of running additional samples or replicates and increasing statistical confidence, mean that uptake of these methods has been minimal. In contrast, in the last 15 years there have been significant advances in the resolution and sensitivity of ion mobility spectrometry, to the point where high-resolution separation of analytes based on their collision cross section approaches chromatographic separation, with minimal loss in sensitivity. Additionally, ion mobility separations can be performed on a chromatographic timescale with little reduction in instrument duty cycle. In this review, we compare ion mobility separation to liquid chromatographic separation, highlight the history of the use of ion mobility separations in metabolomics, outline the current state-of-the-art in the field, and discuss the future outlook of the technology. “Where there is one, you’re bound to divide it. Right in two”, James Maynard Keenan.
DOI：
10.1007/s11306-023-01973-4
发表时间：
2023-02-02
期刊：
Metabolomics : Official journal of the Metabolomic Society
影响因子：
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作者：
通讯作者：
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影响因子：
4.1
作者：
Wickramasekara SI;Zandkarimi F;Morré J;Kirkwood J;Legette L;Jiang Y;Gombart AF;Stevens JF;Maier CS
通讯作者：
Maier CS