Non-thermal atmospheric plasma brush induces HEMA grafting onto dentin collagen.
Non-thermal atmospheric plasma brush induces HEMA grafting onto dentin collagen.
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DOI：
10.1016/j.dental.2014.10.004
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发表时间：
2014-12
期刊：
影响因子：
5
通讯作者：
Wang, Yong
中科院分区：
文献类型：
--
作者：
Chen, Mingsheng;Zhang, Ying;Dusevich, Vladimir;Liu, Yi;Yu, Qingsong;Wang, Yong
非热大气等离子体（NTAP）刷被认为是一种很有前途的技术，以提高牙科界面粘结。然而，主要的增强机制还没有得到很好的识别。本研究探讨了低温等离子体对牙本质胶原薄膜接枝牙本质单体HEMA的影响。将人牙本质切成10 μ m厚的薄膜。在0.5M EDTA溶液中完全脱矿30分钟后，将牙本质胶原膜用水冲洗，风干，用35wt%HEMA水溶液处理。然后在NTAP刷下以不同的时间（1-8分钟）/输入功率（5-15 w）对膜进行等离子体暴露。为了比较，牙本质胶原膜也用上述含有光引发剂的HEMA溶液处理，然后进行光固化。在等离子体曝光或光固化后，将HEMA-胶原膜在去离子水中冲洗，然后通过FTIR光谱和TEM进行检查。FITR结果表明，等离子体暴露可以诱导显着的HEMA接枝到牙本质胶原薄膜。相比之下，光固化导致没有检测到的HEMA与牙本质胶原蛋白的相互作用。定量IR光谱分析（即，1720/3075或749/3075，HEMA/胶原比率）进一步表明，HEMA在等离子体暴露的胶原薄膜上的接枝功效强烈依赖于等离子体的处理时间和输入功率。透射电镜结果表明，等离子体处理没有改变胶原的带状结构。本研究为深入了解非热等离子体处理增强牙体附着力的机制提供了新的思路。NTAP刷可能是一种很有前途的方法，建立树脂单体和牙本质胶原之间的化学键。
Non-thermal atmospheric plasma (NTAP) brush has been regarded as a promising technique to enhance dental interfacial bonding. However, the principal enhancement mechanisms have not been well identified. In this study, the effect of non-thermal plasmas on grafting of HEMA, a typical dental monomer, onto dentin collagen thin films was investigated. Human dentin was sectioned into 10-um-thick films. After total demineralization in 0.5 M EDTA solution for 30 min, the dentin collagen films were water-rinsed, air-dried, treated with 35 wt% HEMA aqueous solution. The films were then subject to plasma-exposure under a NTAP brush with different time (1–8 min) / input power (5–15 w). For comparison, the dentin collagen films were also treated with the above HEMA solution containing photo-initiators, then subject to light-curing. After plasma-exposure or light-curing, the HEMA-collagen films were rinsed in deionized water, and then examined by FTIR spectroscopy and TEM. The FITR results indicated that plasma-exposure could induce significant HEMA grafting onto dentin collagen thin films. In contrast, light-curing led to no detectable interaction of HEMA with dentin collagen. Quantitative IR spectral analysis (i.e., 1720/3075 or 749/3075, HEMA/collagen ratios) further suggested that the grafting efficacy of HEMA onto the plasma-exposed collagen thin films strongly depended on the treatment time and input power of plasmas. TEM results indicated that plasma treatment did not alter collagen’s banding structure. The current study provides deeper insight into the mechanism of dental adhesion enhancement induced by non-thermal plasmas treatment. The NTAP brush could be a promising method to create chemical bond between resin monomers and dentin collagen.
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通过非热，大气等离子体刷对几种牙齿底物进行表面修饰。
DOI：
10.1016/j.dental.2013.05.002
发表时间：
2013-08
期刊：
Dental materials : official publication of the Academy of Dental Materials
影响因子：
--
作者：
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影响因子：
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作者：
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影响因子：
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通讯作者：
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14
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