The Continuing Mystery of Lipid Rafts.
The Continuing Mystery of Lipid Rafts.
复制标题
DOI：
10.1016/j.jmb.2016.08.022
复制
发表时间：
2016-12-04
影响因子：
5.6
通讯作者：
Veatch, Sarah L.
中科院分区：
文献类型：
--
作者：
Levental, Ilya;Veatch, Sarah L.
自近20年前首次形式化以来，脂筏的概念引起了极大的关注和兴趣，也几乎引起了同样多的争议。这场争论可能令人惊讶，因为这个概念本身是直观的：在时间和空间上的划分是生物学中所有尺度上普遍存在的主题，因此应该预料到将细胞膜划分为横向细分。尽管如此，导致区划的物理化学原理及其功能化的分子机制在今天仍然和20年前一样神秘。在此，我们回顾了这一主题的最新文献，尤其是该领域的主要开放问题，包括：(1)什么是最好的工具来分析浮筏在活的膜中的行为；(2)哺乳动物细胞的复杂脂体相对于膜组织的功能是什么；(3)驱动浮筏形成并决定其特性的机制是什么；(4)如何调节浮筏的形成；(5)如何将膜的区隔化整合到细胞信号中。尽管进行了数十年的密集研究，但这个引人注目的领域仍然充满了根本性的问题。
Since its initial formalization nearly twenty years ago, the concept of lipid rafts has generated a tremendous amount of attention and interest, and nearly as much controversy. The controversy is perhaps surprising because the notion itself is intuitive: compartmentalization in time and space is a ubiquitous theme at all scales of biology, and therefore the partitioning of cellular membranes into lateral subdivision should be expected. Nevertheless, the physicochemical principles responsible for compartmentalization and the molecular mechanisms by which they are functionalized remain nearly as mysterious today as they were two decades ago. Herein, we review recent literature on this topic with a specific focus on the major open questions in the field, including: (1) what are the best tools to assay raft behavior in living membranes; (2) what is the function of the complex lipidome of mammalian cells with respect to membrane organization; (3) what are the mechanisms that drive raft formation and determine their properties; (4) how can rafts be modulated; (5) how is membrane compartmentalization integrated into cellular signaling. Despite decades of intensive research, this compelling field remains full of fundamental questions.
登录
查看更多内容
影响因子：
3.4
作者：
Burns, Margaret C.;Nouri, Mariam;Veatch, Sarah L.
通讯作者：
Veatch, Sarah L.
影响因子：
3
作者：
Eckelmann, Jens;Luening, Ulrich
通讯作者：
Luening, Ulrich
影响因子：
4.8
作者：
Chan, Robin B.;Oliveira, Tiago G.;Di Paolo, Gilbert
通讯作者：
Di Paolo, Gilbert
影响因子：
3.4
作者：
Brewster, Robert;Safran, Samuel A.
通讯作者：
Safran, Samuel A.
影响因子：
3.4
作者：
Dietrich, C;Bagatolli, LA;Gratton, E
通讯作者：
Gratton, E