Barley Hv CIRCADIAN CLOCK ASSOCIATED 1 and Hv PHOTOPERIOD H1 Are Circadian Regulators That Can Affect Circadian Rhythms in Arabidopsis.
Barley Hv CIRCADIAN CLOCK ASSOCIATED 1 and Hv PHOTOPERIOD H1 Are Circadian Regulators That Can Affect Circadian Rhythms in Arabidopsis.
复制标题
DOI：
10.1371/journal.pone.0127449
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Webb AA
中科院分区：
文献类型：
--
作者：
Kusakina J;Rutterford Z;Cotter S;Martí MC;Laurie DA;Greenland AJ;Hall A;Webb AA
生物钟调节植物生理和发育的许多方面，这些方面有助于基本的农艺性状。生物钟包含转录反馈环，被认为能产生昼夜节律。在植物界，组成生物钟转录和翻译反馈环的基因有相当大的相似性。生物钟基因的功能特征仅限于少数模式物种。在这里，我们提供了大麦昼夜节律时钟基因Hv昼夜节律相关1(HvCCA1)和Hv PHOTOPERIODH1的功能特征，它们分别与拟南芥昼夜节律时钟相关1(AtCCA1)和伪反应调节7(AtPRR7)最相似。这为了解北欧主要作物物种之一的昼夜节律提供了洞察。通过对大麦昼夜节律和野生型和突变体异源表达的生理分析，我们证明了HvCCA1对AtCCA1具有保守功能。我们发现，Hv光周期H1在拟南芥中具有类似AtPRR7的功能，并且Hv光周期H1突变对光周期和昼夜节律的影响在基因上是可分离的。
Circadian clocks regulate many aspects of plant physiology and development that contribute to essential agronomic traits. Circadian clocks contain transcriptional feedback loops that are thought to generate circadian timing. There is considerable similarity in the genes that comprise the transcriptional and translational feedback loops of the circadian clock in the plant Kingdom. Functional characterisation of circadian clock genes has been restricted to a few model species. Here we provide a functional characterisation of the Hordeum vulgare (barley) circadian clock genes Hv CIRCADIAN CLOCK ASSOCIATED 1 (HvCCA1) and Hv PHOTOPERIODH1, which are respectively most similar to Arabidopsis thaliana CIRCADIAN CLOCK ASSOCIATED 1 (AtCCA1) and PSEUDO RESPONSE REGULATOR 7 (AtPRR7). This provides insight into the circadian regulation of one of the major crop species of Northern Europe. Through a combination of physiological assays of circadian rhythms in barley and heterologous expression in wild type and mutant strains of A. thaliana we demonstrate that HvCCA1 has a conserved function to AtCCA1. We find that Hv PHOTOPERIOD H1 has AtPRR7-like functionality in A. thaliana and that the effects of the Hv photoperiod h1 mutation on photoperiodism and circadian rhythms are genetically separable.
登录
查看更多内容
影响因子：
5.3
作者：
Campoli C;Shtaya M;Davis SJ;von Korff M
通讯作者：
von Korff M
影响因子：
56.9
作者：
Huang, W.;Perez-Garcia, P.;Mas, P.
通讯作者：
Mas, P.
影响因子：
7.4
作者：
Green, RM;Tingay, S;Tobin, EM
通讯作者：
Tobin, EM
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
9.4
作者：
Dodd, AN;Parkinson, K;Webb, AAR
通讯作者：
Webb, AAR