Characterization of immobilized pH gradient formed in capillary based on quantum dots

Characterization of immobilized pH gradient formed in capillary based on quantum dots
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基于量子点的毛细管中形成的固定化 pH 梯度的表征

DOI:
10.1109/iccme.2007.4381903
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发表时间:
2007-05
期刊:
2007 IEEE/ICME International Conference on Complex Medical Engineering
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基于量子点的荧光成像技术,建立了一种新的毛细管固定化pH梯度的荧光成像方法。结果表明,无论从毛细管的哪一端进样,经CIEF后,带正电荷的量子点都集中在同一正电荷区。通过BSA富集实验考察了pH梯度的形成及其在实际分离中的作用,结果表明,与毛细管区带电泳(CZE)相比,该方法检测BSA的灵敏度明显提高。
A new fluorescence imaging method was used for the characterization of the immobilized pH gradient formed in capillary, which was based on charge carried quantum dots (QDs). It was found that the positive charged QDs were concentrated in the same positive region in capillary after CIEF, no matter which terminal of the capillary was used for injection. Furthermore, the formation of the pH gradient and their effect in the practical separation was tested by BSA enrichment experiment, which showing that the sensitivity of detection of BSA was improved remarkably compared with capillary zone electrophoresis (CZE).
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