An autotetraploid linkage map of rose (Rosa hybrida) validated using the strawberry (Fragaria vesca) genome sequence.
An autotetraploid linkage map of rose (Rosa hybrida) validated using the strawberry (Fragaria vesca) genome sequence.
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DOI：
10.1371/journal.pone.0020463
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Zamir D
中科院分区：
文献类型：
--
作者：
Gar O;Sargent DJ;Tsai CJ;Pleban T;Shalev G;Byrne DH;Zamir D
多倍体是植物进化中的一个关键过程，因为它增加了基因冗余和形态复杂性，但由于多体遗传的复杂性，我们只有很少的同源多倍体生物的遗传图谱。一个强大的映射框架是特别重要的多倍体作物物种，包括玫瑰（2n = 4x = 28），其中的目标是研究多等位基因的相互作用，控制性状的植物育种价值。 从花园，桃红色和芳香品种香云（FC）和切玫瑰黄色品种金门（GG）之间的杂交，我们产生了同源四倍体GGFC作图群体由132个个体组成。利用128个序列标记、141个AFLP标记、86个SSR标记和3个形态标记构建了该图谱。7个连锁群被解析为FC（总632 cM）和GG（616 cM），其通过在双亲中分离的标记以及二倍体整合的共有图谱进行验证。野草莓基因组的发布，也属于蔷薇亚科，使我们能够在七个草莓假染色体上放置70个玫瑰测序标记。Rosa和Fragaria之间的同线性很高，估计需要四个主要的易位和六个倒位来将17个非共线标记以相同的顺序放置。基于一个验证的线性顺序的玫瑰标记，我们可以进一步分区的每一个父母到它的四个同源组，从而提供了一个必要的框架，以帮助同源四倍体基因组的测序。
Polyploidy is a pivotal process in plant evolution as it increase gene redundancy and morphological intricacy but due to the complexity of polysomic inheritance we have only few genetic maps of autopolyploid organisms. A robust mapping framework is particularly important in polyploid crop species, rose included (2n = 4x = 28), where the objective is to study multiallelic interactions that control traits of value for plant breeding. From a cross between the garden, peach red and fragrant cultivar Fragrant Cloud (FC) and a cut-rose yellow cultivar Golden Gate (GG), we generated an autotetraploid GGFC mapping population consisting of 132 individuals. For the map we used 128 sequence-based markers, 141 AFLP, 86 SSR and three morphological markers. Seven linkage groups were resolved for FC (Total 632 cM) and GG (616 cM) which were validated by markers that segregated in both parents as well as the diploid integrated consensus map. The release of the Fragaria vesca genome, which also belongs to the Rosoideae, allowed us to place 70 rose sequenced markers on the seven strawberry pseudo-chromosomes. Synteny between Rosa and Fragaria was high with an estimated four major translocations and six inversions required to place the 17 non-collinear markers in the same order. Based on a verified linear order of the rose markers, we could further partition each of the parents into its four homologous groups, thus providing an essential framework to aid the sequencing of an autotetraploid genome.
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