Multifunctional organically modified silica nanoparticles for chemotherapy, adjuvant hyperthermia and near infrared imaging.
Multifunctional organically modified silica nanoparticles for chemotherapy, adjuvant hyperthermia and near infrared imaging.
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DOI：
10.1016/j.colsurfb.2016.07.048
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发表时间：
2016-11-01
影响因子：
5.8
通讯作者：
McGoron, Anthony J.
中科院分区：
文献类型：
--
作者：
Nagesetti, Abhignyan;McGoron, Anthony J.
关键词：
我们报告了一种新型有机改性二氧化硅纳米粒子（Ormosil）系统，能够产生近红外荧光和辅助热疗化疗，用于图像引导癌症治疗。 Ormosil np 负载有化疗药物阿霉素 (DOX) 和花青染料 IR820。 Ormosil 颗粒的平均直径为 51.2 ± 2.4 纳米，表面电荷为 -40.5±0.8 毫伏。通过物理吸附 (FDSIR820) 或共价连接 (CDSIR820) 与 Ormosil 表面上的硅烷醇基团将 DOX 负载到 Ormosil 颗粒上。两种制剂在水性缓冲液中保留了 2 天的 DOX 和 IR820，但与 FDSIR820 (77.0%) 纳米粒子相比，CDSIR820 保留了更多的 DOX (93.2%)。暴露于近红外激光会触发 CDSIR820 释放 DOX。通过卵巢癌细胞（Skov-3）中的反卷积显微镜测定纳米颗粒的摄取。 CDSIR820 定位于细胞溶酶体中，而与 FDSIR820 一起孵育的细胞显示出来自细胞核的 DOX 荧光，表明 DOX 从纳米颗粒基质中泄漏。 FDSIR820 纳米颗粒在 Skov-3 细胞中显示出严重的毒性，而 CDSIR820 颗粒具有与裸（无 DOX 和 IR820）Ormosil 颗粒相同的细胞毒性特征。此外，将 CDSIR820 纳米粒子暴露于 808 纳米的近红外激光会产生热量（从 37°C 升至 43°C），与游离 DOX 处理相比，细胞杀伤力增强。生物分布研究表明CDSIR820纳米颗粒主要存在于网状内皮（RES）系统的器官中。一种基于 Ormosil 二氧化硅纳米粒子的新型多功能治疗诊断探针，用于体内成像以及化疗与辅助热疗 (43°C) 的组合。
We report a novel system of organically modified silica nanoparticles (Ormosil) capable of near infrared fluorescence and chemotherapy with adjuvant hyperthermia for image guided cancer therapy. Ormosil np’s were loaded with a chemotherapeutic, Doxorubicin (DOX) and cyanine dye, IR820. Ormosil particles had a mean diameter of 51.2 ± 2.4 nanometers and surface charge of −40.5±0.8 millivolts. DOX was loaded onto Ormosil particles via physical adsorption (FDSIR820) or covalent linkage (CDSIR820) to the silanol groups on Ormosil surface. Both formulations retained DOX and IR820 over period of 2 days in aqueous buffer, though CDSIR820 retained more DOX (93.2%) compared to FDSIR820 (77.0 %) nanoparticles. Exposure to near infrared laser triggered DOX release from CDSIR820. Uptake of nanoparticles was determined by deconvolution microscopy in ovarian carcinoma cells (Skov-3). CDSIR820 localized in the cell lysosomes whereas, cells incubated with FDSIR820 showed DOX fluorescence from the nucleus indicating leakage of DOX from the nanoparticle matrix. FDSIR820 nanoparticles showed severe toxicity in Skov-3 cells whereas, CDSIR820 particles had the same cytotoxicity profile as Bare (No DOX and IR820) Ormosil particles. Furthermore, exposure of CDSIR820 nanoparticles to Near Infrared laser at 808 nanometers resulted in generation of heat (to 43°C from 37°C) and resulted in enhanced cell killing compared to Free DOX treatment. Bio-distribution studies showed that CDSIR820 nanoparticles were primarily present in the organs of Reticuloendothelial (RES) system. A novel multifunctional theranostic probe based on ormosil silica nanoparticles for in-vivo imaging and combination of chemotherapy with adjuvant hyperthermia (43°C).
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影响因子：
17.1
作者：
Kumar R;Roy I;Ohulchanskky TY;Vathy LA;Bergey EJ;Sajjad M;Prasad PN
通讯作者：
Prasad PN
DOI：
10.1016/j.jconrel.2013.09.013
发表时间：
2013-12-28
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Ernsting MJ;Murakami M;Roy A;Li SD
通讯作者：
Li SD
影响因子：
3.9
作者：
Lei T;Manchanda R;Fernandez-Fernandez A;Huang YC;Wright D;McGoron AJ
通讯作者：
McGoron AJ
影响因子：
3.9
作者：
Fukami, T;Nakasu, S;Matsuda, M
通讯作者：
Matsuda, M
影响因子：
17.1
作者：
Albanese, Alexandre;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.