Sensory afferent and hypoxia-mediated activation of nucleus tractus solitarius neurons that project to the rostral ventrolateral medulla.
Sensory afferent and hypoxia-mediated activation of nucleus tractus solitarius neurons that project to the rostral ventrolateral medulla.
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DOI：
10.1016/j.neuroscience.2010.02.012
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发表时间：
2010-05-05
期刊：
影响因子：
3.3
通讯作者：
Hasser, E. M.
中科院分区：
文献类型：
--
作者：
Kline, D. D.;King, T. L.;Austgen, J. R.;Heesch, C. M.;Hasser, E. M.
脑干的孤束核（nTS）接收感觉传入输入，处理该信息，并将投射发送到负责影响自主神经和呼吸输出的各种大脑区域。nTS将直接投射发送到延髓头端腹外侧（RVLM），这是一个对心肺反射和体内平衡很重要的区域。由于nTS处理的净反射效应最终取决于输出神经元的性质，我们使用电生理和免疫组织化学技术确定了这些RVLM投射nTS神经元的特征。RVLM投射的nTS神经元通过逆行示踪剂鉴定。水平脑干nTS切片的膜片钳分析表明，RVLM投射的nTS细胞表现出恒定的潜伏期孤束诱发的EPSC，这表明它们从内脏传入接受强的单突触接触。在RVLM投射细胞中观察到三种不同的动作电位放电模式，与不同的潜在钾电流相关。急性缺氧3小时激活清醒动物的化学反射后，11.2 ± 1.9%的RVLM投射的nTS神经元被激活，如Fos免疫反应阳性所示。极少数RVLM投射nTS细胞是儿茶酚胺能的。两者合计，这些数据表明，RVLM投射的NTS神经元接受强烈的单突触输入的感觉传入和亚群参与化学反射通路。
The nucleus tractus solitarius (nTS) of the brainstem receives sensory afferent inputs, processes that information, and sends projections to a variety of brain regions responsible for influencing autonomic and respiratory output. The nTS sends direct projections to the rostral ventrolateral medulla (RVLM), an area important for cardiorespiratory reflexes and homeostasis. Since the net reflex effect of nTS processing ultimately depends on the properties of output neurons, we determined the characteristics of these RVLM-projecting nTS neurons using electrophysiological and immunohistochemical techniques. RVLM-projecting nTS neurons were identified by retrograde tracers. Patch clamp analysis in the horizontal brainstem nTS slice demonstrated that RVLM-projecting nTS cells exhibit constant latency solitary tract evoked EPSCs, suggesting they receive strong monosynaptic contacts from visceral afferents. Three distinct patterns of action potential firing, associated with different underlying potassium currents, were observed in RVLM-projecting cells. Following activation of the chemoreflex in conscious animals by three hours of acute hypoxia, 11.2 ± 1.9% of the RVLM-projecting nTS neurons were activated, as indicated by positive Fos-immunoreactivity. Very few RVLM-projecting nTS cells were catecholaminergic. Taken together, these data suggest that RVLM projecting nTS neurons receive strong monosynaptic inputs from sensory afferents and a subpopulation participates in the chemoreflex pathway.
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影响因子：
2.3
作者：
Kline, David D.
通讯作者：
Kline, David D.
DOI：
10.1152/ajpregu.00532.2005
发表时间：
2006-06-01
影响因子：
2.8
作者：
Kantzides, A;Badoer, E
通讯作者：
Badoer, E
影响因子：
2.5
作者：
DEKIN, MS;GETTING, PA
通讯作者：
GETTING, PA
影响因子：
3.3
作者：
Hirooka, Y;Polson, JW;Dampney, RAL
通讯作者：
Dampney, RAL
影响因子：
64.8
作者：
KATZ, LC;BURKHALTER, A;DREYER, WJ
通讯作者：
DREYER, WJ