Genome-Wide Analysis of SQUAMOSA-Promoter-Binding Protein-like Family in Flowering Pleioblastus pygmaeus.
Genome-Wide Analysis of SQUAMOSA-Promoter-Binding Protein-like Family in Flowering Pleioblastus pygmaeus.
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开花苦竹中 SQUAMOSA 启动子结合蛋白样家族的全基因组分析
DOI：
10.3390/ijms232214035
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发表时间：
2022-11-14
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
SQUAMOSA启动子结合蛋白样（SPL）家族在植物生长发育特别是生殖过程中发挥着重要作用。竹子具有特殊的生殖特性，营养期较长，开花时间不确定。然而，SPL基因在竹植物生殖生长中的潜在功能尚不清楚。本研究从观赏矮竹种Pleioblastus pygmaeus中筛选出28个单核苷酸多态性位点。系统发育分析表明，来自8种植物的183个SPLs可划分为9个亚科，28个PpSPLs分布于8个亚科。同源性分析表明，稻瘟病菌与水稻共鉴定出32对同源基因，与毛竹共鉴定出83对同源基因，且Ka/Ks值均<1。MiRNA靶标预测显示，28个PpSPLs中有13个具有与miRNA156互补的识别位点。为了筛选与竹类植物生殖生长有关的SPLs，采用RNA-Seq方法对开花竹和非开花竹不同组织中28个PpSPLs的mRNA丰度进行了分析。此外，8种PpSPLs在开花植物中的相对表达量显著高于非开花植物，RT-qPCR也证实了这一点。结合系统发育分析和同源性分析，鉴定出8个显著差异表达的PpSPLs与生殖过程和花器官发育有关。其中，有4个潜在的靶向mirna156的PpSPLs参与开花过程。本研究的重点是鉴定出28个SPLs，并探索了4个与花相关的关键SPLs，为揭示SPLs在竹植物生殖生长中的潜在功能提供了理论依据。
SQUAMOSA Promoter-Binding Protein-Like (SPL) family is well-known for playing an important role in plant growth and development, specifically in the reproductive process. Bamboo plants have special reproductive characteristics with a prolonged vegetative phase and uncertain flowering time. However, the underlying functions of SPL genes in reproductive growth are undisclosed in bamboo plants. In the study, a total of 28 SPLs were screened from an ornamental dwarf bamboo species, Pleioblastus pygmaeus. Phylogenetic analysis indicates that 183 SPLs from eight plant species can be classified into nine subfamilies, and the 28 PpSPLs are distributed among eight subfamilies. Homologous analysis shows that as many as 32 pairs of homologous genes were found between P. pygmaeus and rice, and 83 pairs were found between P. pygmaeus and Moso bamboo, whose Ka/Ks values are all <1. MiRNA target prediction reveals that 13 out of the 28 PpSPLs have recognition sites complementary to miRNA156. To screen the SPLs involved in the reproductive growth of bamboo plants, the mRNA abundance of the 28 PpSPLs was profiled in the different tissues of flowering P. pygmaeus and non-flowering plants by RNA-Seq. Moreover, the relative expression level of eight PpSPLs is significantly higher in flowering P. pygmaeus than that in non-flowering plants, which was also validated by RT-qPCR. Combined with phylogenetic analysis and homologous analysis, the eight significant, differentially expressed PpSPLs were identified to be associated with the reproductive process and flower organ development. Among them, there are four potential miRNA156-targeting PpSPLs involved in the flowering process. Of significant interest in the study is the identification of 28 SPLs and the exploration of four key flowering-related SPLs from P. pygmaeus, which provides a theoretic basis for revealing the underlying functions of SPLs in the reproductive growth of bamboo plants.
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影响因子：
5.6
作者：
Hu L;Chen W;Yang W;Li X;Zhang C;Zhang X;Zheng L;Zhu X;Yin J;Qin P;Wang Y;Ma B;Li S;Yuan H;Tu B
通讯作者：
Tu B
影响因子：
2.1
作者：
Martin, Ruth C.;Asahina, Masashi;Nonogaki, Hiroyuki
通讯作者：
Nonogaki, Hiroyuki
影响因子：
27.5
作者：
Chao, Lu-Men;Liu, Yao-Qian;Chen, Xiao-Ya
通讯作者：
Chen, Xiao-Ya
影响因子：
11.4
作者：
Fryxell, KJ
通讯作者：
Fryxell, KJ
DOI：
10.16288/j.yczz.16-008
发表时间：
2016-06-01
期刊：
Yichuan
影响因子：
--
作者：
Chang, Jianzhong;Yan, Fengxia;Liu, Qingshan
通讯作者：
Liu, Qingshan