Antioxidant Defense, Oxidative Modification, and Salivary Gland Function in an Early Phase of Cerulein Pancreatitis
Antioxidant Defense, Oxidative Modification, and Salivary Gland Function in an Early Phase of Cerulein Pancreatitis
复制标题
雨蛙胰腺炎早期阶段的抗氧化防御、氧化修饰和唾液腺功能
DOI：
10.1155/2019/8403578
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发表时间：
2019
影响因子：
--
通讯作者：
A. Zalewska
中科院分区：
文献类型：
--
作者：
M. Maciejczyk;Anna Skutnik;Izabela Zieniewska;J. Matczuk;Emilia Domel;D. Waszkiel;M. Żendzian;I. Szarmach;A. Zalewska
急性胰腺炎（AP）是一种以胰腺坏死性炎症改变为特征的多因素疾病。我们的研究是第一个研究评估的自由基的产生，酶和非酶抗氧化剂，氧化损伤，和分泌功能的AP大鼠的唾液腺之间的关系。雄性Wistar大鼠分为对照组（n = 9）和AP组（n = 9）。腹腔注射雨蛙肽诱导AP，并通过较高的血清淀粉酶和脂肪酶证实。我们已经证明，超氧化物歧化酶和谷胱甘肽还原酶的活性，以及还原型谷胱甘肽的浓度，显着降低在AP大鼠的腮腺和颌下腺相比，对照组大鼠。与对照组相比，AP大鼠的腮腺和颌下腺中自由基的产生（二氯二氢荧光素测定）以及NADPH氧化酶和黄嘌呤氧化酶的活性和IL-1β浓度显着升高。在AP大鼠中，我们还发现氧化修饰产物（晚期糖基化终产物和晚期氧化蛋白产物），唾液淀粉酶活性的统计学增加，总蛋白含量显着降低。然而，我们没有显示AP大鼠的唾液腺的组织学检查的细胞凋亡和任何形态学变化。综上所述，雨蛙素诱导的AP加剧了氧自由基的产生，损害了唾液腺的氧化还原平衡，并对这些腺体造成更高的氧化损伤。有趣的是，蛋白质的氧化修饰和抗氧化屏障功能障碍在AP大鼠的下颌下腺中更为明显。
Acute pancreatitis (AP) is a multifactorial disease characterized by necroinflammatory changes of the pancreas. Our study is the first study which evaluated the relationship between the free radical production, enzymatic and nonenzymatic antioxidants, oxidative damage, and secretory function of the salivary glands of AP rats. Male Wistar rats were divided equally into 2 groups: control (n = 9) and AP (n = 9). AP was induced by intraperitoneal injection with cerulein and confirmed by higher serum amylase and lipase. We have demonstrated that the superoxide dismutase and glutathione reductase activities, as well as reduced glutathione concentration, were significantly decreased in both the parotid and submandibular glands of AP rats as compared to the control rats. The production of free radicals evidenced as dichlorodihydrofluorescein assay and the activity of NADPH oxidase and xanthine oxidase and IL-1β concentration were significantly higher in the parotid and submandibular glands of AP rats compared to the controls. In AP rats, we also showed a statistical increase in oxidation modification products (advanced glycation end products and advanced oxidation protein products), salivary amylase activity, and significant decrease in the total protein content. However, we did not show apoptosis and any morphological changes in the histological examination of the salivary glands of AP rats. To sum up, cerulein-induced AP intensifies production of oxygen free radicals, impairs the redox balance of the salivary glands, and is responsible for higher oxidative damage to these glands. Interestingly, oxidative modification of proteins and dysfunction of the antioxidant barrier are more pronounced in the submandibular glands of AP rats.
影响因子：
20.1
作者：
GRIENDLING, KK;MINIERI, CA;ALEXANDER, RW
通讯作者：
ALEXANDER, RW
DOI：
10.1016/0926-6917(94)90011-6
发表时间：
1994-08-03
期刊：
EUROPEAN JOURNAL OF PHARMACOLOGY-ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY SECTION
影响因子：
--
作者：
BONDY, SC;GUO, SX
通讯作者：
GUO, SX